Literature Discussion I                                Zoology 533               Sept 9, 2003

 

Assigned Reading:

Donovan and Gearhart (2001) The end of the beginning for pluripotent stem cells.  Nature Vol 414: 92 ­ 98 

Mitsui et al. (2003)  The Homeoprotein Nanog is Required for Maintenance of Pluripotency in Mouse Epiblast and ES Cells.   Cell Vol 113: 631 ­ 642

 

Note: The class discussions and the questions on the midterms will assume that you have read the complete articles.

 

Reminder:  You are encouraged to discuss the articles and the discussion questions with others.  However, the answers to the assigned questions must be written in your own words (not your friendsą words; not the authorsą words). Submit the answers by e-mail prior to class to both instructors japc@iastate.edu; becraft@iastate.edu  Also, bring a copy of your answers and any additional questions or comments to the discussion sessions.

 

 

Assigned Questions 

To receive full credit, answers must be incomplete sentences. 

 

1. Mouse ES cells

a.  Describe how ES cells are derived, and describe some of the properties of ES cells.

b.  What are the roles of the Stat 3 and Oct 4 (also known as Oct 3/4) transcription factors during ES cell propagation or differentiation?  (Donąt try to write a minireview.  Try to summarize the information in less than half a page.)

 

2.  Nanog

To determine whether Nanog is required during ES cell propagation and during embryonic development, Mitsui et al. łknocked-out˛ the Nanog gene. 

  1. How do ES cells lacking Nanog compare to wild-type ES cells?  What do the authors conclude from this?  Do you agree with their conclusions?  Explain.
  2. How do embryos lacking Nanog compare to wild-type embryos?  What do the authors conclude from this?  Do you agree with their conclusions?  Explain.