XAN-2 (Clone 6F11)

(Only cell products will be distributed.)

INVESTIGATOR

Name Donald S. Sakaguchi (Iowa State University)

Address Department of Zoology and Genetics, Iowa State University, Ames, IA 50011

Phone (515) 294-3112 -----Fax (515) 294-8457 ------E-mail dssakagu@iastate.edu

IMMUNOGEN

Substance

Name Xenopus tadpole optic nerves

Origin dounced fresh or frozen tissue

Chemical Composition

Developmental Stage Xenopus stage 45-53 optic nerves

IMMUNIZATION PROTOCOL

Donor Animal

Species mouse

Strain BALB/c

Sex female

Organ and tissue spleen

Immunization

Dates immunized 12/4/84, 12/20/84, 1/7/85

Amount of antigen 100-200 mg

Route of immunization IP

Adjuvant complete Freund's adjuvant followed be incomplete Freund's adjuvant

FUSION

Date 1/11/85

Myeloma cell line

Species mouse

Designation NS1

MONOCLONAL ANTIBODY

Isotype IgG1

Specificity

Cell binding appears membrane associated

Immunohistology embryonic and mature retinal neurons, does not label photoreceptor outer segments

Antibody competition

Species Specificity Xenopus, does not crossreact with opossum, chick or Rana retina.

ANTIGEN

Chemical properties neural specific

Molecular weight bands at ~140 kD and180-200 kDa (approximate) from frog brain and eye homogenate.

Characterization

Immunoprecipitation

Immunoblotting yes, (XAN-1 is preferred over XAN-3 for immunoblotting)

Purification

Amino acid sequence analysis

Functional effects none observed to date

Immunohistochemistry yes, paraformaldehyde fixed material. labels neurons and processes (XAN-4 comparable to XAN-3 and are preferred over XAN-1 for immunohistochemistry). Dilution: 1:5 - 1:20 for indirect immunofluorescence; 1:500-1000 with ABC Elite kit.

PUBLICATIONS :

Sakaguchi, D.S., Moeller, J.F., Coffman, C.R., Gallenson, N., and Harris, W.A. (1989). Growth cone interactions with a glial cell line from embryonic Xenopus retina. Devel. Biol. 134, 158-174.