Germplasm Enhancement of Maize

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Introduction
Materials and Methods
Results and Discussion
Current Direction

GEM - 2005 Public Cooperator's Report

NOTE: The information in this report is shared cooperatively. The data are not published, but are presented with the understanding that they will not be used in publications without specific consent of the public cooperator.

 

Evaluation of GEM testcrosses for yield and resistance to Aspergillus ear rot and aflatoxin accumulation in grain in the southern U.S.

Clements, M.J.1., Windham G.H.1., Williams, W.P.1.,

Maragos, C.M.2., Blanco, M.H.3., and Goodman, M.M.4.

1.USDA-ARS Corn Host Plant Resistance Research Unit, Mississippi State, MS; 2.USDA-ARS Mycotoxin Research Unit, Peoria, IL; 3.USDA-ARS Plant Introduction, Ames, IA; 4.North Carolina State University, Department of Genetics, Raleigh, NC.

INTRODUCTION

BACKGROUND.  Aflatoxin accumulation in corn (Zea mays L.) grain is a concern within U.S. grain markets every year.  Ideally, aflatoxin accumulation in grain should be managed with inbred sources that contribute genetic resistance to disease and insects, tolerance to environmental stress, and superior agronomics (i.e., standability and yield) to hybrid performance.  Although superior resistance to aflatoxin accumulation in grain, severity of Aspergillus ear rot, and/ or kernel infection by Aspergillus flavus has been identified in publicly available sources, agronomic performance from the majority of these sources as inbred lines per se or in hybrid combination is generally poor.

OBJECTIVE.  Evaluate testcrosses developed with GEM and Holdens’ lines for yield and agronomic quality in Mississippi.  Evaluate a subset of the same testcrosses for resistance to Aspergillus ear rot and aflatoxin in inoculated trials at MS State, MS.  The authors also had hoped to develop testcrosses of GEM lines and 7 Holdens’ lines for future evaluations, however, severe weather and herbicide damage terminated this portion of the project.

MATERIALS AND METHODS

Testcross yield and agronomic evaluation.  The experiment was a randomized complete block with 3 replicates.  Experimental units consisted of one row of each genotype.  Rows were approximately 4 meters in length (±0.5 meter fallow alley), spaced 1 meter apart, and included a maximum of 27 plants.  F1 seed were developed in 2004 with Holden’s lines LH195 or LH210 and 145 GEM lines (designated by pedigree), 9 public inbred lines (Mp420, Mp494, Mp715, Mp80:04, Mp717, NC258, NC298, NC320, and Tx601), or 4 experimental lines from USDA-ARS at, MS State (designated by source).  Three commercial checks (Pioneer Brand P3394, Terral Seeds TV2100, and Dekalb DK697) and the cross LH195/LH210 were included for comparison.  Seed were planted in two locations at MS State, MS, however, one location was lost due to hurricane damage.  Genotypes were evaluated for emergence (number of plants plot-1 two weeks after planting), maturity (modified growing degree units (MGDU) to anthesis and silking), root and stalk lodging (% of plants plot-1), staygreen (% green tissue on plants 65 days after silking), yield (bu A-1), and grain moisture (%).  Grain was harvested from all plots with an Almaco research plot combine.

Testcross ear rot and aflatoxin evaluation.  The experiment was a randomized complete block with 4 replicates.  Experimental units consisted of one row of each genotype.  Rows were approximately 4 meters in length (±0.5 meter fallow alley), spaced 1 meter apart, and included a maximum of 21 plants.  F1 seed was developed in 2004 with Holden’s lines LH195 or LH210 and 16 GEM lines identified as resistant or susceptible in previous work, 5 public inbred lines (Mp494, Mp715, Mp80:04, NC258, NC320, and Tx601), or 10 experimental lines from USDA-ARS at MS State (designated by pedigree).  One commercial check (Terral Seeds TV2100), one susceptible check (GA209/SC212M), one resistant check (Mp313E/Mo18W), and the cross LH195/LH210 were included for comparison.  Seed were planted in one location at MS State, MS.  Primary ears on all plants were inoculated with a propagule suspension of A. flavus Link:Fr.  Three-point-four milliliters of the propagule suspension (1 x 106 propagules ml-1) were injected through husk leaves into the side of the primary ear on all plants at the R2 (blister) growth stage.  Primary ears from all plants were harvested, shucked, dried to approximately 15% grain moisture, and visually rated for severity of Aspergillus ear rot as percentage of the ear with symptoms of disease development.  Grain was shelled from cobs, bulked by experimental unit, and ground.  Concentration of aflatoxin in grain currently is being determined with VICAM AflaTest.

Statistical analyses.  Data were analyzed with analysis of variance (ANOVA) using the general linear models procedure of Statistical Analysis System software (SAS Institute, NC).  Replicates were considered random terms in analyses, while genotypes were considered fixed.  Differences between genotypes were compared with Fisher’s protected least significant difference test (LSD).

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RESULTS AND DISCUSSION

Testcross yield and agronomic evaluation.  Emergence, MGDU to silking, MGDU to anthesis, and root lodging were affected significantly (P < 0.05) by genotype within trials in which LH195 or LH210 was the common tester.  Emergence varied from 7 to 26 plants row-1 (mean 20 plants row-1) and from 7 to 25 plants row-1 (mean 19 plants row-1) in trials within which LH195 (Table 1) or LH210 (Table 2) was the common tester, respectively.  Greatest emergence was observed from hybrids developed with 2250-01_XL370A_S11_F2S4_9214-Blk21/00-sib-B in trials within which LH195 or LH210 was the common tester.  Greatest emergence also was observed from hybrids developed with FS8A(S):S09-362-1-B-B and LH195 and 2132-03_DK888_S11_F2S4_

9187-Blk22/00-sib-B and LH210.  MGDU to silking varied from 1244 to 1528 °D (mean 1412 °D) and from 1214 to 1537 °D (mean 1389 °D) in trials within which LH195 (Table 1) or LH210 (Table 2) was the common tester, respectively.  Earliest and latest GEM lines were DKXL370:N11a20-2-1-B-B-B and 2127-01_DK888_S11_F2S4_9181-Blk21/00-sib-B in the trial within which LH195 was the common tester, and CH05015:N15-184-1-B-B-B and 2146-01_DK888_S11_F2S4_9196-Blk29/00-sib-B in the trial within which LH210 was the common tester.  MGDU to anthesis varied from 1206 to 1566 °D (mean 1389 °D) and from 1206 to 1517 °D (mean 1354 °D) in trials within which LH195 (Table 1) or LH210 (Table 2) was the common tester, respectively.  Earliest and latest GEM lines were DKXL370:N11a20-2-1-B-B-B and UR11003:S0302-937-1-B-B in the trial within which LH195 was the common tester, and CH05015:N12-50-1-B-B-B and 7451-22DK888N11 F2S3 691-Blk/02-B in the trial within which LH210 was the common tester.  Root lodging varied from 0 to 46% of plants (mean 5%) and from 0 to 61% of plants (mean 11%) in trials within which LH195 (Table 1) or LH210 (Table 2) was the common tester, respectively.  Fifty-one and nineteen GEM lines had root lodging scores of 0% in trials within which LH195 or LH210 was the common tester, respectively.

Grain moisture was affected significantly by genotype (P < 0.05) only in trials within which LH195 was the common tester (Table 1).  Grain moisture varied from 11.3 to 14.1% (mean 12.9%).  Of the GEM materials, line CH1S775:S1911b-16-1-B-B-B had the least grain moisture, while line 2142-01_DK888_S11_F2S4_9190-Blk19/00-sib-B had the greatest grain moisture at harvest.

Stalk lodging, staygreen, and yield were not affected significantly by genotype (P > 0.05) in trials within which LH195 or LH210 was the common tester.  Stalk lodging averaged 4 or 10%, Staygreen averaged 10 or 3%, and yield averaged 138 or 144 bu A-1 in trials within which LH195 or LH210 was the common tester, respectively (Tables 1 and 2).

Testcross ear rot and aflatoxin evaluation.  Severity of Aspergillus ear rot was affected significantly (P<0.05) by genotype in trials within which LH195 or LH210 was the common tester.  Severity of Aspergillus ear rot ranged from 2 to 22% (mean 9%) or from 2 to 23% (mean 11%) of the ear rotted in trials within which LH195 or LH210 was the common tester, respectively (Table 3).  Least Aspergillus ear rot was observed on the resistant check, Mo18W/Mp313E.  Six GEM lines had severity of Aspergillus ear rot that did not differ significantly from ear rot on Mo18W/Mp313E in the LH195 or LH210 trial.  GEM lines with severity of Aspergillus ear rot that did not differ significantly from least ear rot in both trials were 2250-01_XL370A_S11_F2S4_9214-Blk21/00-sib-B and 2258-03_XL380_S11_F2S4_71/97_

Bulk/98-sib-B.  Data for aflatoxin concentration in grain from this experiment is not yet available.

CURRENT DIRECTION 

Despite severe weather in 2005, ear to row and recurrent selection programs that incorporate several of the most promising GEM lines and breeding crosses continue through efforts of the Corn Host Plant Resistance Research Unit at Mississippi State.

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 Table 1.  Comparison of testcrosses developed with LH195
Genotype Emergencea. MGDUb. to Silking MGDU to anthesis Root lodging (% of plants) Stalk lodging (% of plants) Stay Green (%)c. Yield (bu A-1)d. Grain moisture (%)
(Cuba164:S2008a-83-1-B-B-B)/LH195 20 1499 1479 4 7 0 107 12.9
(CHIS775:S1911b-120-1-B-B)/LH195 23 1386 1368 0 7 0 133 12.5
(AR13026:S15-68-1-B)/LH195 24 1415 1415 0 9 0 121 12.7
(DKXL370:N11a20-604-1-B)/LH195 22 1441 1378 3 0 38 144 13
(UR10001:S1813-159-3-B)/LH195 17 1469 1461 0 0 0 110 12.4
(AR17056:N2025-685-1-B-B)/LH195 18 1305 1246 2 4 8 144 12.6
(CHIS775:S1911b-327-1-B-B)/LH195 23 1431 1441 0 0 0 124 13.2
(AR03056:N09-250-1-B-B-B)/LH195 21 1368 1305 2 16 3 176 12.6
(AR16026:S1704-153-1-B-B)/LH195 22 1431 1431 0 3 0 125 13.1
(AR16035:S19-161-1-B-B)/LH195 20 1422 1413 0 3 0 144 13.3
(CHIS740:S1411a-783-2-B-B)/LH195 18 1424 1406 8 0 2 119 12.8
(CHIS775:S1911b-439-1-B-B)/LH195 18 1386 1395 0 1 0 114 12.6
(CUBA117:S15-372-1-B-B)/LH195 23 1460 1460 0 5 0 103 12.8
(CUBA164:S1511b-325-1-B-B)/LH195 18 1414 1405 0 5 2 113 12.8
(CUBA164:S1517-163-1-B-B)/LH195 21 1396 1404 1 5 8 112 13.1
(CUBA164:S1517-241-2-B-B)/LH195 14 1396 1323 0 2 5 112 12.6
(CUBA164:S2012-444-1-B-B)/LH195 21 1460 1423 5 2 13 128 12.3
(CUBA164:S2012-966-1-B-B)/LH195 11 1434 1398 13 0 2 78 11.4
(DKB844:N11b-118-1-B-B)/LH195 18 1404 1387 13 5 7 142 12.7
(DKXL212:N11a-139-1-1-B-B)/LH195 22 1397 1350 0 0 15 168 13.3
(DKXL370:N11a20-322-B-B)/LH195 13 1323 1332 2 3 0 116 13
(FS8A(S):S09-362-1-B-B)/LH195 26 1404 1396 4 1 10 108 12.4
(UR01089:S0525-519-1-B-B)/LH195 20 1377 1387 0 0 7 148 13.2
(CUBA164:S1517-82-1-B-B)/LH195 20 1404 1404 0 0 0 139 12.9
(2011-01_SE32_S17_F2S4_9148-Blk22/00-sib-B)/LH195 22 1404 1396 0 8 3 145 13
(2084-02_DK212T_S11_F2S4_9151-Blk38/00-sib-B)/LH195 22 1422 1396 2 8 2 131 12.6
(2086-01_DK212T_S11_F2S4_9154-Blk20/00-sib-B)/LH195 22 1404 1350 2 1 8 146 13.1
(2088-01_DK212T_S11_F2S4_9157-Blk29/00-sib-B)/LH195 21 1436 1436 0 0 3 114 13
(2089-01_DK212T_S11_F2S4_9160-Blk22/00-sib-B)/LH195 20 1461 1423 5 2 10 119 12.5
(2109-01_DK212T_S11_F2S4_63/97_Bulk/97-98-sib-B)/LH195 22 1469 1395 2 0 7 131 12.9
(2111-01_DK212T_S11_F2S4_9166-Blk31/00-sib-B)/LH195 22 1462 1462 7 1 7 129 13
(2112-02_DK212T_S11_F2S4_9169-Blk20/00-sib-B)/LH195 19 1450 1423 2 0 7 124 13.1
(2116-02_DK212T_S11_F2S4_9172-Blk28/00-sib-B)/LH195 21 1450 1424 0 0 3 120 13
(2120-01_DK888_S11_F2S4_9175-Blk28/00-sib-B)/LH195 19 1450 1431 0 13 5 121 13.1
(2127-01_DK888_S11_F2S4_9181-Blk21/00-sib-B)/LH195 22 1528 1510 0 15 0 81 12.2
(2131-01_DK888_S11_F2S4_9184-Blk20/00-sib-B)/LH195 21 1404 1396 0 0 18 151 13.4
(2132-03_DK888_S11_F2S4_9187-Blk22/00-sib-B)/LH195 22 1479 1442 0 0 23 127 13.3
(2142-01_DK888_S11_F2S4_9190-Blk19/00-sib-B)/LH195 17 1488 1450 0 16 2 90 13.9
(2143-02_DK888_S11_F2S4_9193-Blk19/00-sib-B)/LH195 19 1441 1415 2 0 8 108 12.5
(2146-01_DK888_S11_F2S4_9196-Blk29/00-sib-B)/LH195 .e. . . . . . . .
(2150-01_DK888_S11_F2S4_9199-Blk16/00-sib-B)/LH195 24 1460 1460 0 2 2 157 13.1
(2152-02_DK888_S11_F2S4_9202-Blk24/00-sib-B)/LH195 14 1404 1369 0 0 0 80 13.1
(2152-03_DK888_S11_F2S4_9205-Blk24/00-sib-B)/LH195 18 1423 1413 18 0 5 106 13.4
(2156-02_DK888_S11_F2S4_3311-Blk03/00-sib-B)/LH195 22 1442 1396 0 12 8 110 13.3
(2201-01_DKB830_S11_F2S4_9208-Blk27/00-sib-B)/LH195 . . . . . . . .
(2226-02_XL370A_S11_F2S4_9211-Blk25/00-sib-B)/LH195 25 1414 1378 7 0 2 167 12.4
(2228-03_DK370A_S11_F2S4_3358-Blk04/00-sib-B)/LH195 22 1433 1396 0 6 12 135 13.3
(2250-01_XL370A_S11_F2S4_9214-Blk21/00-sib-B)/LH195 26 1414 1405 0 11 18 153 12.6
(2250-02_XL370A_S11_F2S4_3363-Blk03/00-sib-B)/LH195 22 1396 1387 4 9 0 169 12.4
(2253-01_XL370A_S11_F2S4_9220-Blk24/00-sib-B)/LH195 24 1423 1423 0 0 3 166 12.4
(2258-03_XL380_S11_F2S4_71/97_Bulk/98-sib-B)/LH195 21 1499 1481 33 3 7 134 13.1
(2282-01_XL380_S11_F2S4_9226-Blk26/00-sib-B)/LH195 21 1414 1413 0 2 50 136 12.7
(2283-01_XL380_S11_F2S4_9229-Blk20/00-sib-B)/LH195 20 1431 1414 0 7 33 164 12.8
(UR13085:N0215-14-1-B-B-B-B)/LH195 22 1305 1254 13 8 5 144 12.6
(FS8B(T):N11a-322-1-B-B)/LH195 12 1368 1350 18 6 2 116 12.8
(UR11003:S0302-937-1-B-B)/LH195 24 1441 1566 0 3 0 162 12.8
(DK212T N11 F2S2 7431-3-3[3050-003/00]1-)/LH195 21 1396 1378 2 0 2 171 13
(DK888 N11 F2S2 7451-3-1[3291-001/00]1-)/LH195 23 1450 1424 10 11 7 149 12.7
(DK212T N11 F2S2 7431-3-53[3315-003/00]1-)/LH195 20 1450 1441 6 3 12 163 12.8
(DK212T N11 F2S2 7431-3-22[3315-022/00]1-)/LH195 12 1386 1368 6 0 3 132 12.6
(1367-01DK888S11b F1S2 1438-Blk/02-B)/LH195 21 1488 1450 2 5 2 107 12.6
(1937-02DKB844S16 F2S2 1478-Blk/02-B)/LH195 18 1405 1397 12 2 8 161 12.8
(CH05015:N12-50-1-B-B-B)/LH195 23 1378 1323 13 0 28 125 12.6
(CH05015:N12-102-1-B-B-B)/LH195 18 1322 1270 29 4 20 130 12.9
(CH05015:N12-33-1-B-B-B)/LH195 19 1386 1369 7 5 15 124 12.9
(CH05015:N12-7-1-B-B-B)/LH195 22 1387 1369 0 7 10 145 13.1
(FS8B(T):N1802-45-1-1-sib-B-B-B)/LH195 24 1413 1360 1 1 12 172 12.7
(CUBA164:S2008a-23-1-B-B-B)/LH195 22 1386 1351 9 3 0 171 12.6
(CH1S775:S1911b-16-1-B-B-B)/LH195 7 1422 1405 0 0 0 62 11.3
(CUBA164:S2008a-6-1-B-B-B)/LH195 20 1423 1404 0 10 7 132 12.7
(DKXL370:N11a20-31-1-B-B-B)/LH195 22 1359 1296 2 6 22 157 12.5
(UR13085:N0215-11-1-B-B-B)/LH195 20 1386 1351 2 2 23 156 12.8
(FS8B(T):N1802-36-1-B-B-B)/LH195 17 1397 1387 0 0 5 177 12.3
(DKXL370:N11a20-2-1-B-B-B)/LH195 18 1244 1206 0 0 33 151 12.8
(UR13085:N0215-14-1-B-B-B)/LH195 21 1378 1323 0 0 8 122 13.2
(UR13085:N0215-21-1-B-B-B)/LH195 19 1423 1378 0 0 3 134 12.5
(FS8B(T):N1802-35-1-B-B-B)/LH195 21 1359 1342 2 0 15 133 12.6
(CH05015:N15-3-1-B-B-B)/LH195 14 1423 1368 2 0 2 122 12.6
(CH05015:N15-87-1-B-B-B)/LH195 19 1253 1246 2 5 23 149 12.8
(CH05015:N15-82-1-B-B-B)/LH195 22 1368 1333 10 3 30 153 12.6
(CH05015:N15-44-1-B-B-B)/LH195 21 1359 1305 0 8 10 161 12.8
(CH05015:N15-182-1-B-B-B)/LH195 22 1270 1229 3 8 5 157 12.9
(CH05015:N15-184-1-B-B-B)/LH195 . . . . . . . .
(CH05015:N15-143-1-B-B-B)/LH195 21 1323 1279 0 2 7 151 12.6
(CH05015:N15-166-1-B-B-B)/LH195 18 1386 1360 0 2 2 137 13
(CH05015:N15-149-1-B-B-B)/LH195 8 1396 1368 0 0 5 86 12.5
(CH05015:N15-183-1-B-B-B)/LH195 22 1388 1378 2 6 33 142 13.1
(CH05015:N12-20-1-B-B-B)/LH195 20 1405 1361 18 7 12 134 13.3
(CH05015:N12-92-1-B-B-B)/LH195 23 1359 1270 24 3 53 163 12.9
(CH05015:N12-36-1-B-B-B)/LH195 20 1341 1323 10 7 18 142 12.2
(CH05015:N12-18-1-B-B-B)/LH195 23 1334 1305 0 0 23 165 13.4
(CH05015:N12-94-1-B-B-B)/LH195 15 1279 1279 9 9 23 106 12.9
(CH05015:N12-43-1-B-B-B)/LH195 . . . . . . . .
(CH05015:N12-12-1-B-B-B)/LH195 19 1369 1369 43 0 17 134 12.7
(CH05015:N12-131-1-B-B-B)/LH195 . . . . . . . .
(CH05015:N12-12231-1-B-B-B)/LH195 22 1341 1278 0 0 32 142 13.3
(CH05015:N12-140-1-B-B-B)/LH195 21 1395 1378 4 2 5 174 13.1
(CH05015:N12-130-1-B-B-B)/LH195 21 1359 1342 20 0 15 132 13
(FS8B(T):N1802-45-1-1SIB-B-B-B)/LH195 21 1422 1397 0 3 3 147 12.6
(DKXL370:N11a20-31-1-B-B-B)/LH195 23 1368 1368 3 6 22 120 12
(FS8B(T):N1802-36-1-B-B-B)/LH195 25 1415 1386 0 3 13 192 12.3
(UR13085:N0215-2-1-B-B-B)/LH195 21 1386 1333 3 3 2 127 13.4
(CH05015:N15-169-1-B-B-B)/LH195 19 1368 1341 0 0 3 125 12.3
(CH05015:N12-38-1-B-B-B)/LH195 17 1444 1453 13 3 0 149 12.8
(PE001n16F2S2-44)/LH195 22 1488 1479 1 6 12 165 13.5
(2120-01_DK888_S11_F2S4_9175-Blk28/00)/LH195 25 1469 1469 14 15 0 138 13.2
(2152-03_DK888_S11_F2S4_9205-Blk24/00)/LH195 23 1422 1414 32 1 2 119 12.5
(357-7DK888S11b F1S2 1428-Blk/02-B)/LH195 19 1433 1424 2 0 12 114 13.1
(7451-22DK888N11 F2S3 771-Blk/02-B)/LH195 21 1431 1441 5 0 25 162 13.2
(7451-22DK888N11 F2S3 791-Blk/02-B)/LH195 22 1414 1423 0 3 2 169 12.6
(7451-22DK888N11 F2S3 811-Blk/02-B)/LH195 23 1414 1414 0 18 2 157 13
(7541-15 x L380N11 F2S3 661-Blk/02-B)/LH195 24 1396 1360 8 3 42 180 12.8
(7451-22DK888N11 F2S3 691-Blk/02-B)/LH195 23 1460 1460 0 0 23 165 13.2
(7451-22DK888N11 F2S3 701-Blk/02-B)/LH195 23 1450 1415 1 1 5 171 12.9
(7451-22DK888N11 F2S3 711-Blk/02-B)/LH195 20 1450 1422 5 0 17 149 13.1
(7451-22DK888N11 F2S3 721-Blk/02-B)/LH195 18 1452 1416 0 4 10 164 13.2
(7451-22DK888N11 F2S3 751-Blk/02-B)/LH195 22 1441 1433 1 4 8 151 13
(7451-22DK888N11 F2S3 761-Blk/02-B)/LH195 19 1451 1438 0 8 35 172 13.3
(IS740S14 F2S3 49-1-7 2914-Blk/02-B)/LH195 17 1460 1469 4 4 7 104 13
Mp420/LH195 . . . . . . . .
Mp494/LH195 19 1471 1451 6 5 3 151 14.1
Mp715/LH195 19 1517 1507 0 2 3 133 13.6
Mp80:04/LH195 . . . . . . . .
Mp717/LH195 13 1450 1431 10 5 3 101 13.7
NC258/LH195 18 1470 1462 10 0 25 148 13.8
NC298/LH195 21 1467 1452 13 7 10 150 12.9
NC320/LH195 23 1507 1479 0 0 3 158 13.1
Tx601/LH195 20 1469 1442 4 5 15 142 14.1
(2003:142-1)/LH195 . . . . . . . .
(2003:146-1)/LH195 . . . . . . . .
(2003:152-1)/LH195 . . . . . . . .
(2003:160-1)/LH195 . . . . . . . .
(03-04:189-1)/LH195 . . . . . . . .
(03-04:190-1)/LH195 . . . . . . . .
(03-04:191-1)/LH195 . . . . . . . .
(03-04:195-1)/LH195 . . . . . . . .
(03-04:196-1)/LH195 . . . . . . . .
(PW03-04:190-1)/LH195 21 1488 1460 46 12 15 130 12.9
(PW03-04:195-1)/LH195 19 1460 1422 2 5 17 146 13.2
(PW03-04:196-1)/LH195 18 1441 1405 4 5 8 172 13.5
(PW2003:160-1)/LH195 20 1405 1368 16 2 23 140 13.2
DK697 22 1405 1323 0 13 12 177 12.7
P3394 21 1332 1297 3 0 20 157 12.9
TV2100 17 1396 1350 23 2 7 134 12.2
LH195/LH210 16 1405 1396 0 0 5 146 12.6
Mean 20 1412 1389 5 4 10 138 12.9
LSD (α = 0.05)f. 4 59 63.7 16.4 . . . 0.7
Coefficient of variation 12 3 3 199 155 101 16 3
Minimum 7 1244 1206 0 0 0 62 11.3
Maximum 26 1528 1566 46 18 53 192 14.1
                 
a. Number of plants from a possible of 25 in a 4 meter row.     
b. Maturity was determined by calculating modified growing degree units base of 50 ºF (MGDU, ºD) from planting for each experimental unit.   
c. Stay green was estimated visually as percent of green tissue present within a plot 65 days post silking.     
d. Yield to 15.5% grain moisture.     
e. Not evaluated or not an appropriate statistical comparison.     
f. Fisher’s protected least significant difference test.     

 

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 Table 2.  Comparison of testcrosses developed with LH210
Genotype Emergencea. MGDUb. to Silking MGDU to anthesis Root lodging (% of plants) Stalk lodging (% of plants) Stay Green (%)c. Yield (bu A-1)d. Grain moisture (%)
(Cuba164:S2008a-83-1-B-B-B)/LH210 14 1359 1333 3 0 0 118 12.6
(CHIS775:S1911b-120-1-B-B)/LH210 20 1404 1369 0 33 0 116 13
(AR13026:S15-68-1-B)/LH210 14 1387 1350 12 16 0 98 12.4
(DKXL370:N11a20-604-1-B)/LH210 20 1396 1360 7 9 2 133 12.3
(UR10001:S1813-159-3-B)/LH210 18 1423 1404 0 0 0 142 13.1
(AR17056:N2025-685-1-B-B)/LH210 19 1378 1342 0 5 0 151 12.5
(CHIS775:S1911b-327-1-B-B)/LH210 21 1433 1405 9 30 2 136 12.7
(AR03056:N09-250-1-B-B-B)/LH210 20 1332 1305 5 53 2 127 12.7
(AR16026:S1704-153-1-B-B)/LH210 10 1302 1302 33 13 0 116 12.8
(AR16035:S19-161-1-B-B)/LH210 17 1415 1405 32 16 2 97 12.5
(CHIS740:S1411a-783-2-B-B)/LH210 16 1386 1324 2 6 0 134 12.4
(CHIS775:S1911b-439-1-B-B)/LH210 9 1378 1369 12 12 0 119 12.9
(CUBA117:S15-372-1-B-B)/LH210 17 1359 1342 4 2 0 137 12.7
(CUBA164:S1511b-325-1-B-B)/LH210 22 1424 1396 6 5 0 149 12.6
(CUBA164:S1517-163-1-B-B)/LH210 24 1305 1279 7 18 0 170 12.6
(CUBA164:S1517-241-2-B-B)/LH210 21 1377 1333 0 18 2 143 12.6
(CUBA164:S2012-444-1-B-B)/LH210 22 1368 1333 5 2 2 162 13
(CUBA164:S2012-966-1-B-B)/LH210 21 1423 1368 18 1 0 152 12.2
(DKB844:N11b-118-1-B-B)/LH210 15 1404 1378 61 0 0 112 13
(DKXL212:N11a-139-1-1-B-B)/LH210 22 1415 1405 8 0 3 175 12.8
(DKXL370:N11a20-322-B-B)/LH210 22 1414 1360 30 8 3 151 12.5
(FS8A(S):S09-362-1-B-B)/LH210 17 1342 1308 13 9 0 146 12.6
(UR01089:S0525-519-1-B-B)/LH210 17 1263 1246 12 26 3 99 12.6
(CUBA164:S1517-82-1-B-B)/LH210 21 1396 1368 11 32 0 127 13
(2011-01_SE32_S17_F2S4_9148-Blk22/00-sib-B)/LH210 8 1288 1263 4 0 2 112 13
(2084-02_DK212T_S11_F2S4_9151-Blk38/00-sib-B)/LH210 24 1423 1368 6 13 0 154 12.5
(2086-01_DK212T_S11_F2S4_9154-Blk20/00-sib-B)/LH210 23 1469 1442 0 23 0 142 13.2
(2088-01_DK212T_S11_F2S4_9157-Blk29/00-sib-B)/LH210 19 1423 1387 2 4 0 167 13.3
(2089-01_DK212T_S11_F2S4_9160-Blk22/00-sib-B)/LH210 22 1405 1368 6 13 2 170 13.2
(2109-01_DK212T_S11_F2S4_63/97_Bulk/97-98-sib-B)/LH210 20 1415 1351 0 16 0 153 12.8
(2111-01_DK212T_S11_F2S4_9166-Blk31/00-sib-B)/LH210 21 1387 1314 0 9 0 158 13.1
(2112-02_DK212T_S11_F2S4_9169-Blk20/00-sib-B)/LH210 14 1386 1378 0 0 2 137 13.2
(2116-02_DK212T_S11_F2S4_9172-Blk28/00-sib-B)/LH210 22 1413 1351 0 6 0 151 13.1
(2120-01_DK888_S11_F2S4_9175-Blk28/00-sib-B)/LH210 14 1413 1378 5 5 0 157 13.3
(2127-01_DK888_S11_F2S4_9181-Blk21/00-sib-B)/LH210 22 1450 1405 0 68 0 123 12.9
(2131-01_DK888_S11_F2S4_9184-Blk20/00-sib-B)/LH210 23 1527 1460 1 7 0 180 11.8
(2132-03_DK888_S11_F2S4_9187-Blk22/00-sib-B)/LH210 25 1396 1360 0 1 0 177 13.4
(2142-01_DK888_S11_F2S4_9190-Blk19/00-sib-B)/LH210 22 1450 1387 0 1 0 157 13.4
(2143-02_DK888_S11_F2S4_9193-Blk19/00-sib-B)/LH210 23 1424 1395 0 3 0 143 12.9
(2146-01_DK888_S11_F2S4_9196-Blk29/00-sib-B)/LH210 24 1528 1471 7 12 0 184 11.8
(2150-01_DK888_S11_F2S4_9199-Blk16/00-sib-B)/LH210 18 1423 1396 6 18 0 142 13
(2152-02_DK888_S11_F2S4_9202-Blk24/00-sib-B)/LH210 22 1404 1387 0 2 0 158 12.9
(2152-03_DK888_S11_F2S4_9205-Blk24/00-sib-B)/LH210 16 1314 1332 25 0 0 169 12.9
(2156-02_DK888_S11_F2S4_3311-Blk03/00-sib-B)/LH210 9 1413 1378 0 5 0 110 13
(2201-01_DKB830_S11_F2S4_9208-Blk27/00-sib-B)/LH210 21 1415 1387 22 2 0 143 12.1
(2226-02_XL370A_S11_F2S4_9211-Blk25/00-sib-B)/LH210 21 1386 1341 41 2 2 190 12.6
(2228-03_DK370A_S11_F2S4_3358-Blk04/00-sib-B)/LH210 19 1424 1424 35 4 3 138 13.2
(2250-01_XL370A_S11_F2S4_9214-Blk21/00-sib-B)/LH210 25 1404 1369 7 4 2 166 13
(2250-02_XL370A_S11_F2S4_3363-Blk03/00-sib-B)/LH210 23 1368 1324 22 10 0 164 12.8
(2253-01_XL370A_S11_F2S4_9220-Blk24/00-sib-B)/LH210 24 1351 1314 11 2 5 167 12.5
(2258-03_XL380_S11_F2S4_71/97_Bulk/98-sib-B)/LH210 20 1405 1360 8 3 0 162 13.1
(2282-01_XL380_S11_F2S4_9226-Blk26/00-sib-B)/LH210 20 1369 1341 3 0 13 152 12.6
(2283-01_XL380_S11_F2S4_9229-Blk20/00-sib-B)/LH210 .e. . . . . . . .
(UR13085:N0215-14-1-B-B-B-B)/LH210 20 1288 1251 6 44 0 85 13.4
(FS8B(T):N11a-322-1-B-B)/LH210 15 1359 1314 29 0 0 103 12.7
(UR11003:S0302-937-1-B-B)/LH210 20 1378 1333 3 11 0 149 12.7
(DK212T N11 F2S2 7431-3-3[3050-003/00]1-)/LH210 22 1332 1317 3 49 0 116 12.7
(DK888 N11 F2S2 7451-3-1[3291-001/00]1-)/LH210 . . . . . . . .
(DK212T N11 F2S2 7431-3-53[3315-003/00]1-)/LH210 20 1441 1415 7 24 0 144 13
(DK212T N11 F2S2 7431-3-22[3315-022/00]1-)/LH210 22 1441 1414 13 12 0 134 13.2
(1367-01DK888S11b F1S2 1438-Blk/02-B)/LH210 21 1442 1386 0 5 0 162 12.8
(1937-02DKB844S16 F2S2 1478-Blk/02-B)/LH210 21 1333 1324 25 3 3 177 12.7
(CH05015:N12-50-1-B-B-B)/LH210 21 1221 1206 32 3 15 144 12.5
(CH05015:N12-102-1-B-B-B)/LH210 21 1386 1332 61 2 0 124 13.1
(CH05015:N12-33-1-B-B-B)/LH210 20 1332 1323 3 3 15 147 12.9
(CH05015:N12-7-1-B-B-B)/LH210 23 1341 1278 15 10 7 128 12.7
(FS8B(T):N1802-45-1-1-sib-B-B-B)/LH210 17 1414 1378 2 15 0 123 12.8
(CUBA164:S2008a-23-1-B-B-B)/LH210 21 1441 1341 16 0 0 146 12.9
(CH1S775:S1911b-16-1-B-B-B)/LH210 19 1351 1323 0 0 0 151 12.6
(CUBA164:S2008a-6-1-B-B-B)/LH210 24 1395 1360 1 5 2 196 12.9
(DKXL370:N11a20-31-1-B-B-B)/LH210 . . . . . . . .
(UR13085:N0215-11-1-B-B-B)/LH210 22 1386 1342 14 10 5 166 12.7
(FS8B(T):N1802-36-1-B-B-B)/LH210 18 1368 1305 7 24 0 127 12.4
(DKXL370:N11a20-2-1-B-B-B)/LH210 19 1236 1214 0 6 17 139 12.9
(UR13085:N0215-14-1-B-B-B)/LH210 19 1341 1314 10 7 0 151 12.5
(UR13085:N0215-21-1-B-B-B)/LH210 9 1369 1351 13 0 0 112 13.3
(FS8B(T):N1802-35-1-B-B-B)/LH210 16 1341 1287 2 19 0 98 12.6
(CH05015:N15-3-1-B-B-B)/LH210 17 1442 1387 28 5 0 126 12.9
(CH05015:N15-87-1-B-B-B)/LH210 15 1288 1270 24 2 0 126 12.7
(CH05015:N15-82-1-B-B-B)/LH210 . . . . . . . .
(CH05015:N15-44-1-B-B-B)/LH210 17 1254 1214 26 6 2 121 12.4
(CH05015:N15-182-1-B-B-B)/LH210 . . . . . . . .
(CH05015:N15-184-1-B-B-B)/LH210 17 1214 1214 9 6 0 105 12.5
(CH05015:N15-143-1-B-B-B)/LH210 16 1237 1222 5 2 5 129 12.8
(CH05015:N15-166-1-B-B-B)/LH210 16 1359 1314 27 15 0 134 12.9
(CH05015:N15-149-1-B-B-B)/LH210 7 1323 1278 35 0 28 96 12.9