Germplasm Enhancement of Maize

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Cooperator's Meeting
TAC Meeting
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GEM - 1997 Annual Report

1997 Accomplishments

     
  • Over 9000 yield plots were conducted at or coordinated from Ames, and over 7100 conducted at or coordinated from Raleigh.
  • Eighteen breeding crosses were selected for advancement in 1997 based on yield and value-added trait data.
  • Over 3200 topcrossed lines will be available for Corn Belt and Southern yield testing in 1998.
  • Approximately 130 S2 or S3 lines from 50% tropical GEM breeding crosses beat the average of commercial check hybrids in trials analyzed to date.
  • GEM's value-added research capacity expanded with the addition of an NIR to measure starch, oil, and protein composition and an HPLC to measure protein quality. These acquisitions are thanks to Les Lewis and Dwayne Buxton (NIR) and Richard Dunkle and Adrianna Hewings (HPLC).
  • Many 50% tropical GEM breeding crosses as well as advanced S2 and S3 lines had Gray Leaf Spot resistance equal to the most resistant commercial check hybrid. 
  • Significant resistance to Fusarium ear rot was found among advanced progenies of 4 GEM breeding populations; significant resistance to Aspergillus ear rot was also found in 2 of the 4 populations.
  • GEMís capacity for field research in Ames expanded with the acquisition of a Gleaner K combine with three-row corn head (thanks to ISU and Les Lewis) which will be converted to a plot combine in 1998, and the arrival of a 4-row vacuum plot planter (thanks to Richard Dunkle) and a tractor (purchased from GEM funds) in time for planting.
  • GEMís seed storage capacity in Ames expanded with the acquisition of a cold storage unit due to the efforts of Les Lewis.
  • Two successful field days were held: September 16 at the CAD Uthe farm in Ames, IA and September 8 at the University of Wisconsin in Madison.
  • GEM gained three new private cooperators in 1997.
  • Six hundred sixty lines with non-DIMBOA based European corn borer resistance were evaluated as testcrosses for yield in summer 1997, and 200 of the most insect resistant were backcrossed to the Corn Belt recurrent line, in preparation for release.
  • A total of 12 public cooperator projects were partially supported financially by GEM.

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1996 Cooperatorís Meeting, Chicago, IL

 

Introduction was presented by Wilfredo Salhuana (TSG Chair). Annual reports were presented by Linda Pollak (Coordinator), Marty Carson (North Carolina Project Leader), Susan Duvick (Lab Manager), Eric Wellin (Data Manager) who presented the 1996 Yield Testing Data Summary book. Preliminary reports were given by the following public cooperators: John Dudley, John Ayers, Jim Coors, Gary Munkvold, and Jim Hawk. Neil Widstrom described a new aflatoxin test using a genetic mutant. One new TSG members was elected: Hiep Pham of Cargill. Hiep replaces Jim Parks of Wyffels Hybrids whose term expired. We appreciate his service. Dave Harper discussed lobbying issues, and private cooperators discussed issues of yield tests and data analysis. A mid-meeting coffee break gave cooperators an opportunity to network.

Technical Advisory Committee (TAC) Meetings

December 10, 1996, Chicago, IL: Funding decisions for public cooperators were approved. David Topel, Dean of the ISU College of Agriculture, agreed to support GEM with lobbying. Chuck Murphy was an invited guest who discussed suggestions/ideas for lobbying. According to Chuck, GEM is viewed favorably by USDA administrators. David Harper, Randy Holley, and Kevin Montgomery were assigned to rewrite the protocol. When assigning S1 lines to cooperators to advance, Linda Pollak will require the cooperator to advance a minimum of 250 lines. When lines are divided among private cooperators, each private cooperators will send Linda their S2 selections. Linda will recombine them into the synthetic for release, with remnant S2 seed destroyed.

February 23, 1997, Des Moines, IA: Dave Harper with help from other members put materials together in preparation for testifying before the House of Representatives Agriculture, Rural Development, Food and Drug Administration, and Related Agencies Subcommittee on Appropriations on March 12.A lengthy discussion on the protocol was followed by a decision for Dave to write a new protocol, with later approval by TSG and cooperators.

May 29, 1997, Chicago, IL: Protocol for releasing seed was discussed. Lines/synthetics will be released as GEM lines, using the standard Crop Science release publication and USDA release procedures. Primary cooperators involved in the breeding effort will be authors/originators. Seed will be deposited at NSSL (3000k for lines, 7000k for synthetics), and NCRPIS will curate the seed after its release. A discussion was initiated on protocol for seed increase of breeding crosses with insufficient seed for full evaluations and/or breeding. TSG members were assigned public cooperator projects to visit, and encouraged to visit as many yield tests as possible. We decided to develop a five year plan for GEM.

September 17, 1997, Ames, IA: Dean Topel and Ron Cantrell (Head of ISUís Agronomy Department) were invited to discuss interaction between ISU and GEM. Stephen Smith (chair of NPGS Focus Group) was invited to discuss interaction with the Focus Group, and Leslie Lewis was invited to discuss interaction between ARS and GEM. All discussions were extremely beneficial to GEM. The five-year plan is progressing, when further along it will be available on the web site. Public cooperators will be encouraged to present their research at an upcoming NCR-167 or interregional meeting, as well as publishing in refereed scientific journals and in the yearly GEM data summaries. Wilfredo Salhuana presented a progress report on international GEM. All members agreed to proceed on regeneration of promising breeding crosses with insufficient seed supply. 

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Personnel Changes


Eric Wellin resigned as Data Manager effective January 29, 1997.He was replaced by Timothy Johnson who started on July 6, 1997.Mary Runde resigned as Field Technician during August 1996 and was replaced by Randy Ritland who started on December 9, 1996. Nuo (Mack) Shen started as a Research Food Technologist (postdoctoral) on February 2, 1997 to work on quality and stability of oils with altered fatty acid content. Luiz Michelini arrived in Iowa from Brazil on January 6, 1997 to work as a postdoctoral visiting scientist. 

 

Publicity

  • Presentation of Results of 1996 GEM Yield Testing at NCR-167 by Linda Pollak, Des Moines IA, February 24.

  • Article in Spring Corn Farmer.

  • Mentioned in Bob Streitís column in April 11ís Farm News.

  • Presentation of Enhancement of oil quality through introgression by Pamela White at American Oil Chemists Annual Meeting, Seattle WA, May 11-14.

  • Publication of Incorporation of exotic germplasm into elite maize lines: Maximizing favorable effects of the exotic sources by Major Goodman in C. Clark Cockerham memorial issue of J. of Math. Biology.

  • Distribution of Report of U.S. Germplasm Enhancement for Maize Project (US-GEM) Executive Summary by Wilfredo Salhuana.

  • Publication of Thermal properties of corn starch extracted with different blending methods: Microblender and Homogenizer by K.M. Krieger, S.A. Duvick, L.M. Pollak, and P.J. White Cereal Chem. 74:553-555.

  • Featured (cover story) in ARSís September Agricultural Research magazine.

  • Presented laboratory and field tours to the National Plant Germplasm System Customer Focus Group meeting on September 8.

  • Poster display at ISU Agronomy Day on September 11.

  • Press releases issued by American Genetic Resources Alliance, ARS, and ISU in September.

  • Interview of Linda Pollak by Rich Hawkins, WHB Radio, Kansas City on October 6.

  • Interview of Linda Pollak by Colleen Callahan, WMBD Radio, Peoria IL on October 9.

  • Article in October 9ís The Country Today.

  • Interview of Linda Pollak by Mark Steil, Minnesota Public Radio on October 10.

  • Publication of Thermal properties of starch from 62 exotic maize (Zea mays L.) lines grown in two locations by K.-Y. Ng, L.M. Pollak, S.A. Duvick, and P.J. White in Cereal Chem. 74:837-841.

  • Article in Novemberís Soybean Digest.

  • Poster display at Iowa Seed Association annual meeting, November 19-20, Ames, IA.

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1997 Field Days

September 16, 1997, Ames, IA: The field day was held at the Committee for Agricultural Developmentís Uthe Farm. The Central Iowa Field day was held at the same location on September 17-18.Having our field day held in association with the Central Iowa Field Day meant that attendance by commercial people increased significantly over the three days. On September 16 official greetings from USDA-ARS came from Midwest Area Director, Richard Dunkle, Associate Director Adrianna Hewings, and Corn Insects and Crop Genetics Research Leader Leslie Lewis. Official greetings from Iowa State University came from Ron Dean David Topel. Official greetings from GEM came from TSG chair Wilfredo Salhuana. Posters or presentations were given by Craig Abel, Gary Munkvold, Susan Duvick, Darren Jarboe for Iowa Grain Quality Initiative, Suvrat Singh working with Larry Johnson, and Paul Scott. Featured GEM breeding materials were value added trait selections, new breeding crosses, non-DIMBOA corn borer resistant lines, and breeding crosses being advanced to S2 lines, as well as a demonstration of breeding progress in exotic and Corn Belt materials.

September 8, 1997, Madison WI: Jim Coors was the host for this field day which was part of the University of Wisconsin Corn Breeding and Genetics Open House held at the West Madison Experiment Farm. Jimís silage work using GEM breeding materials was highlighted.

Public Cooperatorsí Data


Status of 1996 Public Data: All submitted public cooperator data summaries have been placed in a booklet format. Applicable yield data has been added to our yield trial database, to allow printing along with yield data (for example, ratings of breeding crosses for gray leaf spot are available for printing on yield data analyses). Submitted data from individual cooperators is available upon request. They are on separate sheets of one Excel 6.0 workbook; this file is available to anyone who desires a copy.

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Public GEM Cooperators Supported in 1997

 

Name
$ Funded

Research Supported 

John Dudley
6000
Evaluation of tropical accessions as sources of genes to improve a Corn Belt hybrid for grain yield and disease resistance
Gary Munkvold
5000
Fusarium ear rot resistance screening of GEM germplasm
Paul Williams
3500
Evaluation of breeding crosses for aflatoxin
Paul Scott
2700
Evaluation for protein quality
James Coors
5060
Silage potential of temperate maize germplasm
Margaret Smith
5650
Anthracnose stalk rot resistance from exotic maize germplasm
Craig Abel
5110
Topcrossing of non-DIMBOA European corn borer resistant lines
Jim Hawk (breeding)
5000
Evaluation of 50% Corn Belt:50% LAMP and 75% Corn Belt:25% LAMP accessions for drought resistance
Robert Lambert
5000
Development of GEM corn lines with multiple disease resistance, starch content, and grain yield
Dean Barry, Bruce Hibbard, Larry Darrah
6245
Evaluation and development of GEM materials for European corn borer, western corn rootworm, rind penetrometer, and vertical root pulling resistance
Jim Hawk 
5000
Southern yield tests
Neil Widstrom 
5000
Southern yield tests
Dennis West 
5000
Southern yield tests
ARS Overhead
735
TOTAL
60000

 

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Ames, IA Breeding Program

1997 Iowa:

Breeding Crosses Selfed to S1ís 

Pedigree

# Rows

Reason for Selection

AR03056:N09

133

high yield in 96522 and 23, Bo-Jac only got 299 S1's last winter
Cuba117:S15

133

high yield, high protein (both endosperm and germ protein) in 96121;high protein and oil and low yield (high germ. prot) in 96120
AR16035:S19

133

high yield in 96520, high oil
UR13088:S0607

133

high starch and high protein (high endosperm protein)
CUBA117:S1520

133

high protein high oil low yield (high germ protein)
CUBA164:S1511b

133

high protein
AR13026:S15

133

high protein high oil
FS8B(T):N11a08a

133

high yield in 96533

Total Selfing Rows

1064

 

S1's of Breeding Crosses for Advancement to S2ís
 

Pedigree

# Rows

Reason for Selection

AR16035:S02

676

high protein high oil (81 S2ís selected)
AR16035:S02

80

high oil (from Novartis) 
SCROGP1:N1310

509

high protein high yield in 96132 (50 S2ís selected)
CUBA164:S2008a

558

high yield 96131 (56 S2ís selected)
CUBA164:S15

649

high yield 96120 high oil (85 S2ís selected)
AR01150:N04

233

share with Darrah and Hallauer (from Cargill)(35 S2ís selected)
FS8B(T):N1809

190

share with Cargill and Growmark (from Growmark)(14 S2ís selected)
DKB844:S1601

21

share with NC+ and Hoegemeyer (from NC+) (7 S2ís selected)
CHIS775:N1912

213

share with Wyffels and Gutwein (from Wyffels) (32 S2ís selected)
DKB844:N11b

164

high yield in 96123 high oil, replication of Limagrain (31 S2ís selected)
AR13035:S11b

46

high oil (from Novartis)
AR16021:S08b

50

high oil (from Novartis)
FS8B(T):N11a

71

high oil (from Novartis)
UR10001:S18

64

high oil (from Novartis)
UR13010:S13

57

high oil (from Novartis)
UR13085:N02

42

high oil (from Novartis)
AR16035:S19

77

high oil (from Novartis)

Total S1 Rows

3700

 

 

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Ď97Puerto Rico Nursery:12 rows increasing CIMMYT lines for new breeding crosses, 200 rows for value-added trait work, 43 rows for regeneration of breeding crosses with insufficient seed supply, 160 rows selfing and crosses of high protein lines (for line development, seed increase for poultry feeding study, and heritability studies). Planted November 20.

í97 Puerto Rico Isolations: Stiff Stalk 318 total female rows: 193 S2ís of public cooperators, 86 Ames S2ís, 31 LAMP lines, and 8 new breeding crosses. Non-Stiff Stalk 359 total female rows:115 S2ís of public cooperators, 221 Ames S2ís, 14 LAMP lines, and 9 new breeding crosses.

Southern GEM, Raleigh, NC 

(M. L. Carson, M. M. Goodman, V. A. Brewster)

Yield Trial Plots:2850 plots in NC; 4294 plots with 9 Cooperators (N. Widstrom, USDA Tifton, GA; D. West, U. of Tennessee; J. Hawk, U. Delaware; P. Williams, USDA Mississippi State; C. Poneleit, U. Kentucky; R. Holley, Novartis Seeds, Henderson, KY; T. Morgan, Dekalb Genetics, Mt. Olive, NC; B. Anderson, Pioneer Hybrid Int., Union City, TN; MGR, Angier, NC.Also, special thanks to K. Martin, Pioneer Hybrid Int. for help in harvesting plots at Tifton, GA).

Summer Nursery Plots:350 selfing rows; 1600 topcross rows (isolations + paired rows),1000 observation rows, 1164 Gray leaf spot trial rows, 1122 ear rot (Fusarium + Aspergillus) trial rows, 100 misc. disease trial rows.

Winter Nursery Plots (97-98):835 selfing rows; 115 paired rows (Puerto Rico w/ L. Pollak).

Preliminary Yield Trial Results: Approximately 130 S2 or S3 lines from 50% tropical GEM breeding crosses beat the average of commercial check hybrids in trials analyzed to date (approx. 75% of results are now analyzed).

Preliminary Disease Trial Results: Many 50% tropical GEM breeding crosses as well as advanced S2 and S3 lines had Gray Leaf Spot resistance equal to the most resistant commercial check hybrid. Significant resistance to Fusarium ear rot was found among advanced progenies of 4 GEM breeding populations; Significant resistance to Aspergillus ear rot was also found in 2 the 4 populations.

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1997 Cooperative Nursery Work

Private In-kind Nursery Support - Summer, 1997 

Company Nursery Rows Pledged Rows Will Do  Populations Populations From
Bo-Jac 200 Advance 2 BC's
AR16021:S0908bBR52051:N0412
 
Cargill 250 500 FS8B(T):N1809 Growmark 
DeKalb 500 Remaking S1's CHIS740:S1411a  
FFR Coop 50 Advance 1 BC  DK212T:N11a10   
Gutwein 150 250 CHIS775:N1912 Wyffels
Global Agro 200 Advance 2 BC's AR16035:S19 BR51501:N11a08d  
Golden Harvest 500 Remaking S1's AR16026:S17  
Great Lakes 200 489 UR13085:S1912  Great Lakes 
Growmark 0 250 FS8B(T):N1809 Growmark
Hoegemeyer 300-400  250 DKB844:S1601 NC+
Holdens 500 (1000 Short)  575 UR10001:S1813 Pioneer
ICI 500 349 AR16026:S1719  ICI
Jung Farms 400 Advance 2 BC's
CASH:N1410UR13085:N0215
 
Limagrain 500 163 DKB844:N11b  Limagrain
NC+ 200 250 DKB844:S1601  NC+ 
Novartis 500   From Major  
Pioneer 800 750-800  Cuba164:S2008a Pioneer
Wyffels 250 250 CHIS775:N1912 Wyffels
Public
Darrrah    300 AR01150:N04  DeKalb
Hallauer    250 AR01150:N05  DeKalb
Hawk   <800

676

DKXL212:N11a

BR51501:N11a12

Limagrain

Gutwein and Great Lakes

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Private In-kind Nursery Support -- Winter 1997-98

 
Company
Nursery Rows Pledged
Comments
Bo-Jac
100
Regenerating breeding crosses
Cargill
100
Topcrossing 100 S2's of FS8B(T):N1809 from Cargill Making breeding crosses
DeKalb
150
151 S2's of AR01150:N04 from Darrah, Hallauer, Pollak
FFR Coop
20
Making breeding cross
Golden Harvest
150
Regenerating breeding crosses
Great Lakes
200
Topcrossing 100 S2's of UR13085:S1912 from Great Lakes Regenerating breeding crosses
Growmark
100
Topcrossing 50 S2's of FS8B(T):N1809 from Growmark
Hoegemeyer
50
Topcrossing 50 S2's of DKB844:S1601 from Hoegemeyer
Holdens
200
Topcrossing 115 S2's of UR10001:S1813 from Holdens Regenerating breeding crosses
ICI
100
Topcrossing 140 S2's of AR16026:S1719 from ICI
Limagrain
100
Topcrossing 35 S2's of DKB844:N11b from Limagrain and 31 from Linda 

Regenerating breeding crosses

NC+
100
Topcrossing 50S2's of DKB844:S1601 from NC+ Regenerating breeding crosses
Novartis
100
Materials from Major Goodman 

Making breeding crosses

Pioneer
300
Topcrossing 116 S2's of CUBA164:S2008a from Pioneer Advancing CHIS775:S1911b to S1's
Wyffels
100
Topcrossing 50 S2's of CHIS775:N1912 from Wyffels and 32 from Linda
Crow's
100
Advancing DKXL370:N11a20 to S1's
Pau
Making breeding crosses
Wilson Seeds
100
Making breeding crosses
 

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Breeding Crosses Advanced Summer 1997 and Winter 1997-98 

Breeding Cross

Cooperator

AR03056:N09

Linda Pollak

Cuba117:S15

Linda Pollak

AR16035:S19

Linda Pollak

UR13088:S0607

Linda Pollak

CUBA117:S1520

Linda Pollak

CUBA164:S1511b

Linda Pollak

AR13026:S15

Linda Pollak

FS8B(T):N11a08a

Linda Pollak

AR16021:S0908b

Bo-Jac

BR52051:N0412

Bo-Jac

DK212T:N11a10

FFR

AR16035:S19

Global Agro

CASH:N1410

Jung Seed Genetics

UR13085:N0215

Jung Seed Genetics

DREP150:N20

Robert Lambert

FS8A:S09

Richard Pratt

CHIS775:S1911b

Pioneer

DKXL370:N11a20

Crows

 

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1997 Cooperative Yield Testing 

In all, there were 25 experiments ranging from four to eight replications, with a total of 168.Those experiments with eight replications had one as disease observation. 

Plots with Private Cooperators 6146
Plots with Public Cooperators 1644
Plots in Ames 1301
TOTAL MIDWEST YIELD PLOTS 9091
Plots in disease observations 1301

1997 GEM Yield Test Entries 

50% tropical breeding crosses 38
25% tropical breeding crosses
68
50% temperate breeding crosses
12
25% temperate breeding crosses
483
Tropical backcrosses
600
TOTAL ENTRIES
1201

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Private Cooperator Yield Trials

Experiment

Cooperator Making Topcross

%Exotic

Tester

Zone

Number of Entries

Number of Replications

97520

Holdens

50%

SS

temperate

107

5

97521

Holdens

50%

SS

temperate

107

5

97522

Holdens

50%

SS

temperate

102

5

97523

Holdens

50%

SS

temperate

101

5

97540

Holdens

25%

nSS

temperate

55

7

97541

Holdens

25%

nSS

temperate

58

7

97542

Holdens

25%

nSS

temperate

57

7

97543

Holdens

25%

nSS

temperate

73

7

97544

Holdens

25%

nSS

temperate

57

7

97550

Holdens

25%

SS

temperate

71

7

97551

Holdens

25%

SS

temperate

70

7

97552

Holdens

25%

SS

temperate

53

7

97553

Holdens

25%

SS

temperate

53

7

97554

Holdens

25%

SS

temperate

53

7

97600

Golden Harvest

Both

nSS

Both

25

8

97562

Pioneer

Both

nSS

both

25

6

97602

Cargill

Both

SS

Both

20

8

97603

Holdens

Both

SS

Both

20

8

97630

Golden Harvest

25%

nSS

Both

22

8

97561

Pioneer

Both

nSS

Both

22

6

97632

Holdens

25%

SS

Both

25

8

97633

Holdens

25%

SS

Both

25

8

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Summary of Yield Trial 1997

 

Experiment Cooperators Making Topcross Tester Type Number of Entries  Number of Replications 
22 4 13-SS & 9-nSS 1201 150

Data Summary Book

The GEM Project Data Summary provides a complete overview of GEM results throughout the year. The 1997 GEM Data Summary book will contain yield data from all trials held in the United States both public and private. Public cooperator summaries and data will be also be featured in the GEM. The book will be available at the NCR-167 meetings in February.

 

Laboratory Report

  • Fatty acid analysis is completed for 1996 and 1997 Fast Track lines (368 and 76 S3 ears, respectively) and for AR16035:S02 (678 S1 ears).
  • Protein, oil and starch composition completed for AR16035:S02 (678 S1 ears).
  • Protein, oil and starch composition completed for SCROGP:N1310 (498 S1 ears).
  • Starch extraction for single kernels completed for 1997 Fast Track lines. 
  • Thermal analysis 50%completed for 1997 Fast Track lines.
  • Starch extraction for bulk kernels partially completed for 1997 Fast Track lines.
  • Perstorp 6500 NIRSystem near infrared spectrometer purchased and delivered.
  • Waters High Performance Liquid Chromatograph December delivery date expected
  • Identified a high protein (16.9%) and high oil (6.0%) line (AR16035:S02-519).
  • Identified high starch (75%) line (AR16035:S02-535).
  • Identified high oil (6.5%) line (AR16035:S02-36).
  • Low enthalpy of starch gelatinization (2.5cal/g, target <2.5 cal/g), CHIS775:S1911b-2-1-2.
  • Narrow range of starch gelatinization (4.50C, target <50C), Cuba164:S008a-23-1-3.
  • Large peak height index of starch gelatinization (1.5, target >1.2), Cuba164:S2008a-23-1-3.
  • Low enthalpy of starch retrogradation (1.1 cal/g, target >1.0cal/g), CHIS775:S1911b-37-1-1.
  • Large percent retrogradation (83%, target >80%), Cuba164:S2008a-23-1-2.

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Oil Quality

Table 1: Fatty Acid Compositions of Corn Belt Checks 

Entry Palmitic % Stearic %  Oleic %  Linoleic % Linolenic % Tot Sats % 
Pioneer 3394  11.3 
1.6
26.3
59.3
1.5
12.9
Pioneer 3489 
8.6
2.5
26.8
60.1
1.1
11.1
B73
10.0
2.1
30.0
56.7
1.2
12.1
Mo17
9.9
2.0
20.1
65.3
0.7
11.9
Fast Track  8.1 - 15.5 
1.1 - 2.8
17.5 - 46.5
39.2 - 68.6
0.6 - 1.7
9.7 - 17.1

 

Table 2. Fatty Acid Compositions of AR16035:S02 S1 lines (ranges) 

Fatty Acid
Composition (%)
Ear Number
Palmitic Acid 9.4 

15.0 

433

535 

Stearic Acid
1.1

2.8

209

512

Oleic Acid
21.1

43.2

511

207

Linoleic Acid
43.4

64.1

207

214

Linolenic Acid
0.7

1.8

575

321

Total Saturated Fatty Acid
11.0

16.9

433

535

 

Table 3. Fatty Acid Compositions of 1996 Fast Track lines (ranges)  

Fatty Acid
Composition (%)
Pedigree
GC Number
Palmitic Acid
8.1

15.5

CH05015:N1206-35-1

GUADO5:N0613-4-1

2106

2087

Stearic Acid

 

1.1

2.8

DK844:N1117-3-1

DKB830:S1117-35-1

1989

1912

Oleic Acid
17.5

46.5

FS8B(T):N1802-35-1

AR17026:N1019-15-1

2075

2138

Linoleic Acid

 

39.2

68.6

AR17026:N1019-15-1

AR13035:S1101-43-1

2138

2150

Linolenic Acid

 

0.6

1.7

DK844:N1117-30-1

AR16026:S1704-42-1

1996

2121

Total Saturated Fatty Acid

 

9.7

17.1

CH05015:N1206-35-1

GUADO5:N0613-4-1

2106

2087

 

Table 4. Fatty Acid Compositions for winter 96 Fast track S3 lines (ranges) 

Fatty Acid
Composition (%)
Pedigree
GC Number
Palmitic Acid

 

9.0

15.7

BR52051:S1713-1-1-1

CUBA164:S2008a-6-1-2

4017

3984

Stearic Acid
1.3

3.0

DK212T:S0610-16-1-2

BR52051:S1713-1-1-1

3969

4017

Oleic Acid

 

19.0

39.8

CHIS775:S1911b-16-1-2

BR52051:S1713-1-1-2

4011

4018

Linoleic Acid

 

46.5

65.1

CHIS775:S1911b-13-1-3

GUAT209:S1317-21-1-1

3951

3995

Linolenic Acid
0.8

1.5

BR52051:S1713-1-1-2

CUBA164:S2008a-6-1-4

4018

3986

Total Saturated Fatty Acid

 

11.6

17.5

DK212T:S0610-25-1-1

DK212T:S0610-10-1-1

3974

3962

 

Composition of Whole Grain

Table 5. NIR Analysis 1(ranges) 

Sample

Protein

Oil

Starch

Density

AR16035:S02

6.1 - 16.9%

3.4 - 6.5%

64.2 - 75.0%

1.23 - 1.35

SCROGP:N1310

5.6 - 14.2%

2.9 - 5.5%

65.9 - 74.3%

1.21 - 1.47

B73

10.9

3.2

72.2

1.29

Mo17

11.9

3.4

70.2

1.30

Pioneer 3394

11.0

3.3

70.7

1.31

Pioneer 3489

8.6

4.6

70.3

1.25

1Values based on a zero percent moisture (dry matter basis) except for density, Which is based on 15% moisture basis.

Important to note in AR16035:S02 ear 519 had 16.9% Protein and 6.0% oil composition. Ear 36 had 6.5% oil and ear 535 had 75% starch compositions.

Starch Quality: Thermal Analysis

 

Table 6. Target Values for Starch Thermal Properties

TpG ( peak onset of gelatinization)
Below 60įC
DHG (enthalphy of gelatinization)
Less than2.5cal/g or more than 4cal/g
RnG (range of gelatinization)
Less than 5įC or greater than 15įC
PHI (peak height index)
Above 1.2
DHR (enthalpy of retrogradation)
Less than 1cal/g
%R (percent retrogradation)
Less than 20% or greater than 80%

 

Table 7. Starch Thermal Properties for Winter 1996 Fast Track S3 lines (ranges) 

 

Value

Pedigree
TpG

64.1

69.8

CHIS775:S1911b-2-1-3

CUBA164:S20089-23-1-3

DHG

 

2.5

3.7

CHIS775:S1911b-2-1-2

CUBA164:S20089-23-1-3

RnG

 

4.8

11.6

CUBA164:S20089-23-1-3

CHIS775:S1911b-13-1-3

PHI

 

0.5

0.5

0.5

0.5

1.5

CHIS775:S1911b-2-1-1

CHIS775:S1911b-2-1-2

CHIS775:S1911b-2-1-3

CHIS775:S1911b-13-1-3

CUBA164:S20089-23-1-3

DHR

 

2.6

1.1

CUBA164:S20089-23-1-2

CHIS775:S1911b-37-1-1

%R

 

38.7

83.0

CHIS775:S1911b-37-1-1

CUBA164:S20089-23-1-2

 

Table 8. Thermal Properties for Corn Belt Checks 

Entry

TpG

RnG

DHG

DHR

%R

PHI

Sigma
65.1

9.1

2.9

1.1

38.8

0.7

Mo17
70.4

5.7

2.9

1.3

40.0

1.1

Pioneer 3394
64.1

11.1

2.7

1.2

48.2

0.5

Pioneer 3489
64.5

10.9

3.0

1.5

51.8

0.5

 

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