Text from Keeling (©1998)
Many of the key enzymes of starch biosynthesis have been cloned from a
few plant species. Most notably from maize endosperm, rice endosperm, barley endosperm, potato tuber and pea embryo.
LOCUS MZEWISHR2 7320 bp ds-DNA PLN 28-JUN-1993
DEFINITION Zea mays wild type shrunken-2 allele, exons 1-16.
ACCESSION M81603
KEYWORDS .
SOURCE Zea mays leaves DNA.
ORGANISM Zea mays
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 7320)
AUTHORS Hannah,L.C. and Shaw,J.R.
TITLE Genomic nucleotide sequence of a wild-type shrunken-2 allele of Zea
mays
JOURNAL Plant Physiol. 98, 1214-1216 (1992)
STANDARD full automatic
COMMENT NCBI gi: 168654
FEATURES Location/Qualifiers
exon 1021..1082
/note="putative"
exon 1521..1745
exon 2222..2344
exon 2629..2799
exon 3036..3125
exon 3217..3303
exon 3388..3443
exon 3546..3639
exon 3805..3917
exon 3986..4058
exon 4217..4297
exon 4463..4549
exon 4620..4724
exon 6546..6653
exon 6735..6795
exon 6912..7287
source 1..7320
/organism="Zea mays"
/sequenced_mol="DNA"
/tissue_type="leaves"
BASE COUNT 1992 A 1463 C 1384 G 2481 T
ORIGIN
1 TGATGCTTTT TCCTGGGCAG GGAGAGCTAT GAGACGTATG TCCTCAAAGC CACTTTGCAT
61 TGTGTGAAAC CAATATCGAT CTTTGTTACT TCATCATGCA TGAACATTTG TGGAAACTAC
121 TAGCTTACAA GCATTAGTGA CAGCTCAGAA AAAAGTTATC TCTGAAAGGT TTCATGTGTA
181 CCGTGGGAAA TGAGAAATGT TGCCAACTCA AACACCTTCA ATATGTTGTT TGCAGGCAAA
241 CTCTTCTGGA AGAAAGGTGT CTAAAACTAT GAACGGGTTA CAGAAAGGTA TAAACCACGG
301 CTGTGCATTT TGGAAGTATC ATCTATAGAT GTCTGTTGAG GGGAAAGCCG TACGCCAACG
361 TTATTTACTC AGAAACAGCT TCAACACACA GTTGTCTGCT TTATGATGGC ATCTCCACCC
421 AGGCACCCAC CATCACCTAT TCACCTATCT CTCGTGCCTG TTTATTTTCT TGCCCTTTCT
481 GATCATAAAA AATCATTAAG AGTTTGCAAA CATGCATAGG CATATCAATA TGCTCATTTA
541 TTAATTTGCT AGCAGATCAT CTTCCTACTC TTTACTTTAT TTATTGTTTG AAAAATATGT
601 CCTGCACCTA GGGAGCTCGT ATACAGTACC AATGCATCTT CATTAAATGT GAATTTCAGA
661 AAGGAAGTAG GAACCTATGA GAGTATTTTT CAAAATTAAT TAGCGGCTTC TATTATGTTT
721 ATAGCAAAGG CCAAGGGCAA AATCGGAACA CTAATGATGG TTGGTTGCAT GAGTCTGTCG
781 ATTACTTGCA AGAAATGTGA ACCTTTGTTT CTGTGCGTGG GCATAAAACA AACAGCTTCT
841 AGCCTCTTTT ACGGTACTTG CACTTGCAAG AAATGTGAAC TCCTTTTCAT TTCTGTATGT
901 GGACATAATG CCAAAGCATC CAGGCTTTTT CATGGTTGTT GATGTCTTTA CACAGTTCAT
961 CTCCACCAGT ATGCCCTCCT CATACTCTAT ATAAACACAT CAACAGCATC GCAATTAGCC
1021 ACAAGATCAC TTCGGGAGGC AAGTGTGATT TCGACCTTGC AGCCACCTTT TTTTGTTCTG
1081 TTGTAAGTAT ACTTTCCCTT ACCATCTTTA TCTGTTAGTT TAATTTGTAA TTGGGAAGTA
1141 TTAGTGGAAA GAGGATGAGA TGCTATCATC TATGTACTCT GCAAATGCAT CTGACGTTAT
1201 ATGGGCTGCT TCATATAATT TGAATTGCTC CATTCTTGCC GACAATATAT TGCAAGGTAT
1261 ATGCCTAGTT CCATCAAAAG TTCTGTTTTT TCATTCTAAA AGCATTTTAG TGGCACGCAA
1321 TTTTGTCCAT GAGGGAAAGG AAATCTGTTT TGGTTACTTT GCTTGAGGTG CATTCTTCAT
1381 ATGTCCAGTT TTATGGAAGT AATAAACTTC AGTTTGGTCA TAAGATGTCA TATTAAAGGG
1441 CAAACATATA TTCAATGTTC AATTCATCGT AAATGTTCCC TTTTTGTAAA AGATTGCATA
1501 CTCATTTATT TGAGTTGCAG GTGTATCTAG TAGTTGGAGG AGATATGCAG TTTGCACTTG
1561 CATTGGACAC GAACTCAGGT CCTCACCAGA TAAGATCTTG TGAGGGTGAT GGGATTGACA
1621 GGTTGGAAAA ATTAAGTATT GGGGGCAGAA AGCAGGAGAA AGCTTTGAGA AATAGGTGCT
1681 TTGGTGGTAG AGTTGCTGCA ACTACACAAT GTATTCTTAC CTCAGATGCT TGTCCTGAAA
1741 CTCTTGTAAG TATCCACCTC AATTATTACT CTTACATGTT GGTTTACTTT ACGTTTGTCT
1801 TTTCAAGGGA AATTTACTGT ATTTTTTGTG TTTTGTGGGA GTTCTATACT TCTGTTGGAC
1861 TGGTTATTGT AAAGATTTGT TCAAATAGGG TCATCTAATA ATTGTTTGAA ATCTGGGAAC
1921 TGTGGTTTCA CTGCGTTCAG GAAAAAGTGA ATTATTGGTT ACTGCATGAA TAACTTATGG
1981 AAATAGACCT TAGAGTTGCT GCATGATTAT CACAAATCAT TGCTACGATA TCTTATAATA
2041 GTTCTTTCGA CCTCGCATTA CATATATAAC TGCAACTCCT AGTTGCGTTC AAAAAAAAAA
2101 ATGCAACTCT TAGAACGCTC ACCAGTGTAA TCTTTCCTGA ATTGTTATTT AATGGCATGT
2161 ATGCACTACT TGTATACTTA TCTAGGATTA AGTAATCTAA CTCTAGGCCC CATATTTGCA
2221 GCATTCTCAA ACACAGTCCT CTAGGAAAAA TTATGCTGAT GCAAACCGTG TATCTGCTAT
2281 CATTTTGGGC GGAGGCACTG GATCTCAGCT CTTTCCTCTG ACAAGCACAA GAGCTACGCC
2341 TGCTGTAAGG GATAACACTG AACATCCAAC GTTGATTACT CTATTATAGT ATTATACAGA
2401 CTGTACTTTT CGAATTTATC TTAGTTTTCT ACAATATTTA GTGGATTCTT CTCATTTTCA
2461 AGATACACAA TTGATCCATA ATCGAAGTGG TATGTAAGAC AGTGAGTTAA AAGATTATAT
2521 TTTTTGGGAG ACTTCCAGTC AAATTTTCTT AGAAGTTTTT TTGGTCCAGA TGTTCATAAA
2581 GTCGCCGCTT TCATACTTTT TTTAATTTTT TAATTGGTGC ACTATTAGGT ACCTGTTGGA
2641 GGATGTTACA GGCTTATTGA TATCCCTATG AGTAACTGCT TCAACAGTGG TATAAATAAG
2701 ATATTTGTGA TGAGTCAGTT CAATTCTACT TCGCTTAACC GCCATATTCA TCGTACATAC
2761 CTTGAAGGCG GGATCAACTT TGCTGATGGA TCTGTACAGG TGATTTACCT CATCTTGTTG
2821 ATGTGTAATA CTGTAATTAG GAGTAGATTT GTGTGGAGAG AATAATAAAC AGATGCCGAG
2881 ATTCTTTTCT AAAAGTCTAG ATCCAAAGGC ATTGTGGTTC AAAACACTAT GGACTTCTAC
2941 CATTTATGTC ATTACTTTGC CTTAATGTTC CATTGAATGG GGCAAATTAT TGATTCTACA
3001 AGTGTTTAAT TAAAAACTAA TTGTTCATCC TGCAGGTATT AGCGGCTACA CAAATGCCTG
3061 AAGAGCCAGC TGGATGGTTC CAGGGTACAG CAGACTCTAT CAGAAAATTT ATCTGGGTAC
3121 TCGAGGTAGT TGATATTTTC TCGTTTATGA ATGTCCATTC ACTCATTCCT GTAGCATTGT
3181 TTCTTTGTAA TTTTGAGTTC TCCTGTATTT CTTTAGGATT ATTACAGTCA CAAATCCATT
3241 GACAACATTG TAATCTTGAG TGGCGATCAG CTTTATCGGA TGAATTACAT GGAACTTGTG
3301 CAGGTATGGT GTTCTCTTGT TCCTCATGTT TCACGTAATG TCCTGATTTT GGATTAACCA
3361 ACTACTTTTG GCATGCATTA TTTCCAGAAA CATGTCGAGG ACGATGCTGA TATCACTATA
3421 TCATGTGCTC CTGTTGATGA GAGGTAATCA GTTGTTTATA TCATCCTAAT ATGAATATGT
3481 CATCTTGTTA TCCAACACAG GATGCATATG GTCTAATCTG CTTTCCTTTT TTTTCCCTTC
3541 GGAAGCCGAG CTTCTAAAAA TGGGCTAGTG AAGATTGATC ATACTGGACG TGTACTTCAA
3601 TTCTTTGAAA AACCAAAGGG TGCTGATTTG AATTCTATGG TTAGAAATTC CTTGTGTAAT
3661 CCAATTCTTT TGTTTTCCTT TCTTTCTTGA GATGAACCCC TCTTTTAGTT ATTTCCATGG
3721 ATAACCTGTA CTTGACTTAT TCAGAAATGA TTTTCTATTT TGCTGTAGAA TCTGACACTA
3781 AAGCTAATAG CACTGATGTT GCAGAGAGTT GAGACCAACT TCCTGAGCTA TGCTATAGAT
3841 GATGCACAGA AATATCCATA CCTTGCATCA ATGGGCATTT ATGTCTTCAA GAAAGATGCA
3901 CTTTTAGACC TTCTCAAGTA ATCACTTTCC TGTGACTTAT TTCTATCCAA CTCCTAGTTT
3961 ACCTTCTAAC AGTGTCAATT CTTAGGTCAA AATATACTCA ATTACATGAC TTTGGATCTG
4021 AAATCCTCCC AAGAGCTGTA CTAGATCATA GTGTGCAGGT AAGTCTGATC TGTCTGGAGT
4081 ATGTGTTCTG TAAACTGTAA ATTCTTCATG TCAAAAAGTT GTTTTTGTTT CCAGTTTCCA
4141 CTACCAATGC ACGATTTATG TATTTTCGCT TCCATGCATC ATACATACTA ACAATACATT
4201 TTACGTATTG TGTTAGGCAT GCATTTTTAC GGGCTATTGG GAGGATGTTG GAACAATCAA
4261 ATCATTCTTT GATGCAAACT TGGCCCTCAC TGAGCAGGTA CTCTGTCATG TATTCTGTAC
4321 TGCATATATA TTACCTGGAA TTCAATGCAT AGAATGTGTT AGACCATCTT AGTTCCATCC
4381 TGTTTTCTTC AATTAGCTTA TCATTTAATA GTTGTTGGCT AGAATTTAAA CACAAATTTA
4441 CCTAATATGT TTCTCTCTTC AGCCTTCCAA GTTTGATTTT TACGATCCAA AAACACCTTT
4501 CTTCACTGCA CCCCGATGCT TGCCTCCGAC GCAATTGGAC AAGTGCAAGG TATATGTCTT
4561 ACTGAGCACA ATTGTTACCT GAGCAAGATT TTGTGTACTT GACTTGTTCT CCTCCACAGA
4621 TGAAATATGC ATTTATCTCA GATGGTTGCT TACTGAGAGA ATGCAACATC GAGCATTCTG
4681 TGATTGGAGT CTGCTCACGT GTCAGCTCTG GATGTGAACT CAAGGTACAT ACTCTGCCAA
4741 TGTATCTACT CTTGAGTATA CCATTTCAAC ACCAAGCATC ACCAAATCAC ACAGAACAAT
4801 AGCAACAAAG CCTTTTAGTT CCAAGCAATT TAGGGTAGCC TAGAGTTGAA ATCTAACAAA
4861 ACAAAAGTCA AAGCTCTATC ACGTGGATAG TTGTTTTCCA TGCACTCTTA TTTAAGCTAA
4921 TTTTTTGGGT ATACTACATC CATTTAATTA TTGTTTTATT GCTTCTTCCC TTTGCCTTTC
4981 CCCCATTACT ATCGCGTCTT AAGATCATAC TACGCACTAG TGTCTTTAGA GGTCTCTGGT
5041 GGACATGTTC AAACCATCTC AATCGGTGTT GGACAAGTTT TTCTTGAATT TGTGCTACAC
5101 CTAACCTATC ACGTATGTCA TCGTTTCAAA CTCGATCCTT CCTGTATCAT CATAAATCCA
5161 ATGCAACATA CGCATTTATG CAACATTTAT CTGTTGAACA TGTCATCTTT TTGTAGGTTA
5221 ACATTATGCA CCATACAATG TAGCATGTCT AATCATCATC CTATAAAATT TACATTTTAG
5281 CTTATGTGGT ATCCTCTTGC CACTTAGAAC ACCATATGCT TGATGCCATT TCATCCACCC
5341 TGCTTTGATT CTATGGCTAA CATCTTCATT AATATCCTCG CCTCTCTGTA TCATTGGTCC
5401 TAAATATGGA AATACATTCT TTCTGGGCAC TACTTGACCT TCCAAACTAA CGTCTCCTTT
5461 GCTCCTTTCT TGTGTGTAGT AGTACCGAAG TCACATCTCA TATATTCGGT TTTAGTTCTA
5521 CTAAGTCCCG GGTTCGATCC CCCTCAGGGG TGAATTTCGG GCTTGGTAAA AAAAATCCCC
5581 TCGCTGTGTC CCGCCCGCTC TCGGGGATCG ATATCCTGCG CGCCACCCTC CGGCTGGGCA
5641 TTGCAGAGTG AGCAGTTGAT CGGCTCGTTA GTGATGGGGA GCGGGGTTCA AGGGTTTTCT
5701 CGGCCGGGAC CATGTTTCGG TCTCTTAATA TAATGCCGGG AGGGCAGTCT TTCCCTCCCC
5761 GGTCGAGTTT TAGTTCTACC GAGTCTAAAA CCTTTGGACT CTAGAGTCCC CTGTCACAAC
5821 TCACAACTCT AGTTTTCTAT TTACTTCTAC CTAGCGTTTA TTAATGATCA CTATATCGTC
5881 TGTAAAAAGC ATACACCAAT GTAATCCCCT TGTATGTCCC TTGTAATATT ATCCATCACA
5941 AGAAAAAAAG GTAAGGCTCA AAGTTGACTT TTGATATAGT CCTATTCTAA TCGAGAAGTC
6001 ATCTGTATCT TCGTCTCTTG TTCGAACACT AGTCACAAAA TTTTTTGTAC ATGTTCTTAA
6061 TGAGTCCAAC GTAATATTCC TTGATATTTT GTCATAAGCC CTCATCAAGT CAATGAAAAT
6121 CACGTGTAGG TCCTTCATTT GTTCCTTATA CTGCTCCATC ACTTGTCTCA TTAAGAAAAT
6181 CTCTCTCATA GTTAACCTTT TGGCATGAAA CAAAATCACA CAGAAGTTGT TTCCTTTTTT
6241 TAAGATCCCA CACAAAAGAG GTTTGATCTA AGGAATCTGG ATCCCTGACA GGTTTATCAA
6301 AATCCTTTGT GTTTTTCTTA AAACTGAATA TTCCTCCAGC TTCTAGTATT GATGTAATAT
6361 TCAATCTGTT TAGCAAGTGA ACACCTTGGT TCTTGTTGTT ACTGTACCCC CCCCCCCCCC
6421 CCCCCCCCGA GGCCCAGATT ACCACGACAT GAATACAAGA ATATTGAACC CAGATCTAGA
6481 GTTTGTTTGT ACTGTTGAAA ATCGGTGACA ATTCATTTTG TTATTGCGCT TTCTGATAAC
6541 GACAGGACTC CGTGATGATG GGAGCGGACA CCTATGAAAC TGAAGAAGAA GCTTCAAAGC
6601 TACTGTTAGC TGGGAAGGTC CCAGTTGGAA TAGGAAGGAA CACAAAGATA AGGTGAGTAT
6661 GGATGTGGAA CCACCGGTTA GTTCCCAAAA ATATCACTCA CTGATACCTG ATGGTATCCT
6721 CTGATTATTT TCAGGAACTG TATCATTGAC ATGAATGCTA GGATTGGGAA GAACGTGGTG
6781 ATCACAAACA GTAAGGTGAG CGAGCGCACC TACATGGGTG CAGAATCTTG TGTGCTCATC
6841 TATCCTAATT CGGTAATTCC TATCCAGCGC TAGTCTTGTG ACCATGGGGC ATGGGTTCGA
6901 CTCTGTGACA GGGCATCCAA GAGGCTGATC ACCCGGAAGA AGGGTACTAC ATAAGGTCTG
6961 GAATCGTGGT GATCTTGAAG AATGCAACCA TCAACGATGG GTCTGTCATA TAGATCGGCT
7021 GCGTGTGCGT CTACAAAACA AGAACCTACA ATGGTATTGC ATCGATGGAT CGTGTAACCT
7081 TGGTATGGTA AGAGCCGCTT GACAGAAAGT CGAGCGTTCG GGCAAGATGC GTAGTCTGGC
7141 ATGCTGTTCC TTGACCATTT GTGCTGCTAG TATGTACTGT TATAAGCTGC CCTAGAAGTT
7201 GCAGCAAACC TTTTTATGAA CCTTTGTATT TCCATTACCT GCTTTGGATC AACTATATCT
7261 GTCATCCTAT ATATTACTAA ATTTTTACGT GTTTTTCTAA TTCGGTGCTG CTTTTGGGAT
//
LOCUS ZMSHRUN2 1867 bp DNA 19-APR-1994
XX
AC S48563;
XX
DT 16-FEB-1993 (Rel. 34, Created)
DT 16-FEB-1993 (Rel. 34, Last updated, Version 1)
XX
DE Sh2=shrunken-2 locus [maize, mRNA Partial, 1867 nt]
XX
KW .
XX
OS Unknown
OC Unclassified.
XX
RN [1]
RP 1-1867
RA Bhave M.R., Lawrence S., Barton C., Hannah L.C.;
RT "Identification and molecular characterization of shrunken-2 cDNA
RT clones of maize.";
RL Plant Cell 2:581-588(1990).
XX
CC This entry [NCBI gibbsq 118333] was created by the journal scanning
CC component of NCBI/GenBank at the National Library of Medicine. This
CC sequence comes from Fig. 4.
XX
FH Key Location/Qualifiers
FH
FT CDS 2..1570
FT /partial
FT /note="Nucleotide sequence shown in Fig. 4 is missing one
FT line (60 bp) beginning at position 1561;
FT mismatches(80,88,89,107,108,265)
FT insertions(519-526;526-541). Description: shrunken-2 locus
FT endosperm ADP-glucose pyrophosphorylase subunit homolog;
FT For the protein sequence (NCBI gibbsq 118334): Method:
FT conceptual translation supplied by author. This sequence
FT comes from Fig. 5. Author-given protein sequence is in
FT conflict with the conceptual translation"
FT /gene="Sh2"
FT /codon_start=1
XX
BASE COUNT 531 A 367 C 435 G 534 T
ORIGIN
1 GAATTCGGGA GGCAAGTGCG ATTTTGATCT TGCAGCCACC TTTTTTTGTT CTGTTGTGTA
61 TCTAGTAGTT GGAGGAGATA TGCAGTTTGC ACTTGCATTG GACACGAACT CAGGTCCTCA
121 CCAGATAAGA TCTTGTGAGG GTGATGGGAT TGACAGGTTG GAAAAATTAA GTATTGGGGG
181 CAGAAAGCAG GAGAAAGCTT TGAGAAATAG GTGCTTTGGT GGTAGAGTTG CTGCAACTAC
241 ACAATGTATT CTTACCTCAG ATGCTTGTCC TGAAACTCTT CATTCTCAAA CACAGTCCTC
301 TAGGAAAAAT TATGCTGATG CAAACCGTGT ATCTGCGATC ATTTTGGGCG GAGGCACTGG
361 ATCTCAGCTC TTTCCTCTGA CAAGCACAAG AGCTACGCCT GCTGTACCTG TTGGAGGATG
421 TTACAGGCTT ATTGATATCC CTATGAGTAA CTGCTTCAAC AGTGGTATAA ATAAGATATT
481 TGTGATGAGT CAGTTCAATT CTACTTCGCT TAACCGCCAT ATTCATCGTA CATACCTTGA
541 AGGCGGGATC AACTTTGCTG ATGGATCTGT ACAGGTATTA GCGGCTACAC AAATGCCTGA
601 AGAGCCAGCT GGATGGTTCC AGGGTACAGC AGACTCTATC AGAAAATTTA TCTGGGTACT
661 CGAGGATTAT TACAGTCACA AATCCATTGA CAACATTGTA ATCTTGAGTG GCGATCAGCT
721 TTATCGGATG AATTACATGG AACTTGTGCA GAAACATGTC GAGGACGATG CTGATATCAC
781 TATATCATGT GCTCCTGTTG ATGAGAGCCG AGCTTCTAAA AATGGGCTAG TGAAGATTGA
841 TCATACTGGA CGTGTACTTC AATTCTTTGA AAAACCAAAG GGTGCTGATT TGAATTCTAT
901 GAGAGTTGAG ACCAACTTCC TGAGCTATGC TATAGATGAT GCACAGAAAT ATCCATACCT
961 TGCATCAATG GGCATTTATG TCTTCAAGAA AGATGCACTT TTAGACCTTC TCAAGTCAAA
1021 ATATACTCAA TTACATGACT TTGGATCTGA AATCCTCCCA AGAGCTGTAC TAGATCATAG
1081 TGTGCAGGCA TGCATTTTTA CGGGCTATTG GGAGGATGTT GGAACAATCA AATCATTCTT
1141 TGATGCAAAC TTGGCCCTCA CTGAGCAGCC TTCCAAGTTT GATTTTTACG ATCCAAAAAC
1201 ACCTTTCTTC ACTGCACCCC GATGCTTGCC TCCGACGCAA TTGGACAAGT GCAAGATGAA
1261 ATATGCATTT ATCTCAGATG GTTGCTTACT GAGAGAATGC AACATCGAGC ATTCTGTGAT
1321 TGGAGTCTGC TCACGTGTCA GCTCTGGATG TGAACTCAAG GACTCCGTGA TGATGGGAGC
1381 GGACATCTAT GAAACTGAAG AAGAAGCTTC AAAGCTACTG TTAGCTGGGA AGGTCCCGAT
1441 TGGAATAGGA AGGAACACAA AGATAAGGAA CTGTATCATT GACATGAATG CTAGGATTGG
1501 GAAGAACGTG GTGATCACAA ACAGTAAGGG CATCCAAGAG GCTGATCACC CGGAAGAAGG
1561 CGTCATATAG ATCGGCTGCG TTTGCGTCTA CAAAACAAGA ACCTACAATG GTATTGCATC
1621 GATGGATCGT GTAACCTTGG TATGGTAAGA GCCGCTTGAC AGGAAGTCGA GCTTCGGGCG
1681 AAGATGCTAG TCTGGCATGC TGTTCCTTGA CCATTTGTGC TGCTAGTATG TACCTGTTAT
1741 AAGCTGCCCT AGAAGTTGCA GCAAACCTTT TTATGAACCT TTGTATTTCC ATTACCCTGC
1801 TTTGGATCAA CTATATCTGT CAGTCCTATA TATTACTAAA TTTTTAAACA AACCAGATTC
1861 CGGAAAA
//