Text from Keeling (©1998)
Many of the key enzymes of starch biosynthesis have been cloned from a
few plant species. Most notably from maize endosperm, rice endosperm, barley endosperm, potato tuber and pea embryo.
LOCUS ZMU18908 2712 bp mRNA PLN 06-MAR-1996
DEFINITION Zea mays Su1p (Sugary1) mRNA, partial cds.
ACCESSION U18908
NID g758725
KEYWORDS .
SOURCE maize.
ORGANISM Zea mays
Eukaryotae; mitochondrial eukaryotes; Viridiplantae;
Charophyta/Embryophyta group; Embryophyta; Magnoliophyta;
Liliopsida; Poales; Poaceae; Zea.
REFERENCE 1 (bases 1 to 2712)
AUTHORS James,M.G., Robertson,D.S. and Myers,A.M.
TITLE Characterization of the maize gene sugary1, a determinant of starch
composition in kernels
JOURNAL Plant Cell 7 (4), 417-429 (1995)
MEDLINE 95290995
REFERENCE 2 (bases 1 to 2712)
AUTHORS Myers,A.M.
TITLE Direct Submission
JOURNAL Submitted (19-DEC-1994) Alan M. Myers, Biochemistry and Biophysics,
Iowa State University, 2110 Molecular Biology Building, Ames, IA
50011, USA
FEATURES Location/Qualifiers
source 1..2712
/organism="Zea mays"
/db_xref="taxon:4577"
/clone="lambda clone H-4; subclones pMJ67 and pMJ68; PCR
product clone pMJ125"
/clone_lib="lambda gt11 cDNA library from R. Schmidt,
Dept. of Biology, University of California at San Diego,
La Jolla, CA 92093, USA"
/chromosome="4S"
/tissue_type="endosperm"
/dev_stage="20 days after pollination"
gene 1..2457
/gene="Sugary1"
CDS <1..2457
/gene="Sugary1"
/note="similar to Pseudomonas sp. isoamylase, Swiss-Prot
Accession Number P26501"
/codon_start=1
/product="Su1p"
/db_xref="PID:g758726"
/translation="RLVTHSTRTHYLIGQSQTNWAPSPPLPLPMAQKLPCVSSPRPLL
AVPAGRWRAGVRGRPNVAGLGRGRLSLHAAAARPVAEAVQAEEDDDDDDEEVAEERFA
LGGACRVLAGMPAPLGATALRGGVNFAVYSSGASAASLSLFAPGDLKADRVTEEVPLD
PLLNRTGNVWHVFIHGDELHGMLCGYRFDGVFAPERGQYYDVSNVVVDPYAKAVVSRG
EYGVPAPGGSCWPQMAGMIPLPYNKFDWQGDLPLGYHQKDLVIYEMHLRGFTKHNSSK
TKHPGTYIGAVSKLDHLKELGVNCIELMPCHEFNELEYFSSSSKMNFWGYSTINFFSP
MARYSSSGIRDSGCGAINEFKAFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGI
DNSTYYMLAPKGEFYNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASI
LTRGCSLWDPVNVYGSPMEGDMITTGTPLVAPPLIDMISNDPILGNVKLIAEAWDAGG
LYQEGQFPHWNVWSEWNGKYRDTVRQFIKGTDGFAGAFAECLCGSPQLYQAGGRKPWH
SIGFVCAHDGFTLADLVTYNSKYNLSNGEDFRDGENHNLSWNCGEEGEFASLSVRRLR
KRQMRNFFVCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKEEQSSDL
YRFCRLMTEFRKECESLGLEDFPTSERLKWHGHQPGKPDWSEASRFVAFTMKDETKGE
IYVAFNTSHLPVVVGLPERSGFRWEPVVDTGKEAPYDFLTDGLPDRAVTVYQFSHFLN
SNLYPMLSYSSIILVLRPDV"
BASE COUNT 637 a 644 c 745 g 686 t
ORIGIN
1 cgtctcgtca cacactccac tcgaacgcac tacttgatcg gccaaagcca aacgaactgg
61 gctccctccc ctccacttcc tctccccatg gcgcagaagc tcccctgcgt ctcgtcgccg
121 cgcccgctgc tcgccgtgcc cgcgggccgg tggcgcgccg gcgtgcgggg ccggcccaat
181 gtggcgggac tggggcgggg gcggttgtct ctccacgccg ccgccgcgcg gcccgtggcc
241 gaggcggtgc aggcggagga ggacgacgac gacgacgacg aggaggtggc cgaggagagg
301 ttcgcgctgg gcggcgcgtg ccgggtgctc gcgggaatgc ccgcgccgct cggcgccacc
361 gcgctccgcg gcggtgtcaa cttcgccgtc tactccagcg gtgcctccgc cgcgtcgctg
421 agcctcttcg ctcccggcga cctcaaggcg gatagggtga ccgaggaggt gcccctcgat
481 cccctgctca accgaacggg aaacgtgtgg cacgtgttca tccacgggga cgagctgcac
541 ggcatgctct gcggatacag gttcgatggc gtgttcgccc ctgagcgcgg acagtactac
601 gatgtgtcca acgttgtggt ggatccatac gctaaggcag tggtaagccg aggtgaatat
661 ggtgtgcctg cgcctggtgg tagttgttgg cctcaaatgg ctggtatgat ccctcttccc
721 tataataagt ttgattggca aggtgaccta ccccttgggt accatcagaa ggaccttgtc
781 atatatgaaa tgcatttgcg tggattcaca aagcacaact caagcaagac aaaacaccca
841 ggaacttaca ttggtgctgt gtcaaagctt gaccatctaa aggaacttgg agtgaactgt
901 atagagctaa tgccctgcca tgagttcaat gagctagagt acttcagctc ctcttcgaag
961 atgaacttct ggggatattc cacaataaat tttttctcac caatggcaag atattcttca
1021 agtggcataa gagactctgg atgtggtgcc ataaatgaat ttaaagcttt tgtaagggag
1081 gcccacaaac ggggaattga ggtgatcatg gatgttgtct tcaatcatac agctgaaggt
1141 aatgagaaag gcccaatatt atcctttagg gggatagata atagtacata ctacatgctt
1201 gcacctaagg gagagtttta taattattct ggttgtggaa ataccttcaa ttgtaatcat
1261 cctgtagtcc gtgaatttat agtggattgc ttgagatact gggtaacaga aatgcatgtt
1321 gatggttttc gttttgacct tgcatctata ctgaccagag gatgcagtct atgggatcca
1381 gttaatgtgt atggaagtcc aatggaaggt gacatgatta cgacagggac acctcttgtt
1441 gccccaccac ttattgacat gattagcaat gacccaattc ttggaaatgt caagctcatt
1501 gctgaagcat gggatgcagg aggtctctat caagaaggtc agtttcctca ctggaacgtt
1561 tggtcagagt ggaatggaaa gtatcgcgat accgtgcgtc agttcatcaa aggcacagat
1621 ggatttgctg gtgcttttgc tgaatgccta tgtggaagtc cacagttata ccaggcaggg
1681 gggaggaagc cttggcacag tatcggcttt gtatgtgcac acgatggatt tacactggct
1741 gatttggtca catacaatag caagtacaac ttgtcaaatg gtgaggactt cagagatggg
1801 gaaaatcata atcttagctg gaattgtggg gaggaaggag aatttgcaag tctgtcagtc
1861 cgaagattaa ggaagaggca aatgcgcaat ttctttgttt gtcttatggt ttctcaggga
1921 gttccaatgt tctacatggg cgatgaatat ggtcacacaa agggagggaa caacaatacg
1981 tactgccatg accattatgt caattatttc cgttgggata agaaggaaga acaatcctct
2041 gatttgtaca gattctgccg tctcatgacc gaattccgca aagaatgtga atctcttggc
2101 cttgaggact tcccgacttc agaacggttg aaatggcacg gtcatcagcc cgggaagcct
2161 gactggtcag aggcaagccg attcgttgcc ttcaccatga aggacgaaac caaaggcgag
2221 atctacgtgg ccttcaacac cagtcacctt ccggtggttg ttgggcttcc agagcgctct
2281 gggttccgat gggagccggt ggtggacacc ggcaaggagg caccatatga cttcctcacc
2341 gatggcctgc cagatcgtgc tgtcaccgtc taccagttct ctcatttcct caactccaat
2401 ctctatccta tgctcagcta ctcctccatc atccttgtat tgcgccctga tgtctgaaag
2461 aagcagatac aatagagtat actatagcgg ttgttctcta ggctgtagca tgcagtggaa
2521 actggaaaat gttggggttg ctctgttgtc ggtagtttac atgcgcatgt cggtatgtgt
2581 acataaagct ggtggatctc agttctcaga tcggactcga gacggcaaaa ccattgccag
2641 ttggctggtt ctctgaagtt ttgtttggtg taaagaaatg gtggtccatc atctactctt
2701 tttttttttt tt
//