Welcome to the Pohl Group Homepage

• Jaipuri, F. A.; Pohl, N. L.* Fluorous-tag Assisted Solution-Phase Synthesis of Linear and Branched Mannose Oligomers, Org. Biomol. Chem. 2008, online.

• Park, G.; Ko, K.-S.; Zakharova, A.; Pohl, N. L.* Mono- Vs. Di-fluorous Tagged Glucosamines for Iterative Oligosaccharide Synthesis. J. Fluorine Chem. 2008, online. (special issue)

• Pohl, N.*; Schwarz, K. Polymer-Supported Reagents and ¹H-¹⁹F NMR Couplings: The Synthesis of 2-Fluoroacetophenone. J. Chem. Educ. 2008, 85, 834-835.

• Pohl, N. L.* Fluorous Tags Catching on Microarrays. Angew. Chem. Int. Ed. 2008, 47, 3868-3870. (invited review)

• Hui, Z.; Brokman, S.; Fang, N.; Pohl, N.; Yeung, E.* Linkage Position and Residue Identification of Disaccharides by Tandem MS and Linear Discriminant Analysis, Rapid Commun. Mass Spectrom. 2008, 22, 1579-1586.

• Jaipuri, F. A.; Collet, B. Y. M.; Pohl, N. L.* Synthesis and Quantitative Evaluation of Glycero-D-manno-heptose Binding to Concanavalin A by Fluorous-tag Assistance. Angew. Chem. Int. Ed. 2008, 47, 1707-1710.

• Chen, G.-S.; Pohl, N. L.* Synthesis of Fluorous Tags for Incorporation of Reducing Sugars into a Quantitative Microarray Platform. Org. Lett. 2008, 10, 785-788.

• Mizanur, R. M.; Pohl, N. L.* A thermostable promiscuous glucose-1-phosphate uridyltransferase from Helicobacter pylori for the synthesis of nucleotide sugars. J. Mol. Catal. B: Enzymatic, 2008, 50, 13-19.

• Yu, Y.; Mizanur, R. M.; Pohl, N. L.* Glycosidase activity profiling for bacterial identification by a chemical proteomics approach. Biocatal. Biotransform. 2008, 26, 25-31.

• Mizanur, R. M.; Griffin, A. K. K.; Pohl, N. L.* Recombinant production and biochemical characterization of a hyperthermostable alpha-glucan/maltodextrin phosphorylase from Pyrococcus furiosus. Archaea, 2007, 2, 169-176.

• Mizanur, R. M.; Pohl, N. L.* Cloning and characterization of a heat-stable CMP-N-acylneuraminic acid synthetase from Clostridium thermocellum. Appl. Microbiol. Biotechnol. 2007, 76, 827-834.

• Pohl, N. L.* Carbohydrate Microarrays and Fluorous-Phase Synthesis: Interfacing Fluorous-Phase Tags with the Direct Formation of Glycoarrays. In Current Fluoroorganic Chemistry: New Synthetic Directions, Technologies, Materials, and Biological Applications; Soloshonok, V. A.; Mikami, K.; Yamazaki, T.; Welch, J. T.; Honek, J. F., Eds. ACS Symposium Series 949; American Chemical Society: Washington, DC, 2007, pp. 261-270. (invited book chapter)

• Mamidyala, S. K.; Ko, K.-S.; Jaipuri, F. A.; Park, G.; Pohl, N. L.* Noncovalent Fluorous Interactions for the Synthesis of Carbohydrate Microarrays. J. Fluorine Chem. 2006, 127, 571-579. (special issue)

• Choi, D. W.; Do, Y. S.; Zea, C. J.; McEllistrem, M. T.; Lee, S.-W.; Semrau, J. D.; Pohl, N. L.; Kisting, C. J.; Scardino, L. L.; Hartsel, S. C.; Boyd, E. S.; Gessey, G. G.; Riedel, T. P.; Shafe, P. H.; Kranski, K. A.; Tritsch, J. R.; Antholine, W. W.; DiSpirito, A. A.* Spectral and Thermodynamic Properties of Ag(I), Au(III), Cd(II), Co(II), Fe(III), Hg(II), Mn(II), Ni(II), Pb(II), U(IV), and Zn(II) Binding by Methanobactin from Methylosinus trichosporium OB3b. J. Inorg. Biochem. 2006, 100, 2150-2161. (special issue)

• Wacker, M.; Feldman, M. F.; Callewaert, N.; Kowarik, M.; Clarke, B. R.; Pohl, N. L.; Hernandez, M.; Vines, E. D.; Valvano, M. A.; Whitfield, C.; Aebi, M.* Substrate Specificity of Bacterial Oligosaccharyltransferase Suggests a Common Transfer Mechanism for the Bacterial and Eukaryotic Systems . Proc. Natl Acad. Sci., USA, 2006, 103, 7088-7093.

• Pohl, N. L.* Building a Bridge to New Antibiotics. ACS Chem. Biol. 2006, 1, 14-16. (invited commentary)

• Pohl, N. L.* Array methodology singles out pathogenic bacteria. Nature Chem. Biol. 2006, 2, 125-126. (invited commentary)

• Choi, D. W.; Zea, C. J; Do, Y. S.; Semrau, J. D.; Antholine, W. E.; Hargrove, M. S.; Pohl, N. L.; Boyd, E. S.; Geesey, G. G.; Hartsel, S. C.; Shafe, P. H.; McEllistrem, M. T.; Kisting, C. J.; Campbell, D.; Rao, V.; de la Mora, A. M.; DiSpirito, A. A.* Spectral, Kinetic, and Thermodynamic Properties of Cu(I)- and Cu(II)-binding by Methanobactin from Methylosinus trichosporium OB3b. Biochemistry, 2006, 45, 1442-1453.

• Pohl, N.* Acyclic Peptide Inhibitors of Amylases. Chem. Biol. 2005, 12, 1257-1258. (invited commentary)

• Ko, K.-S.; Jaipuri, F. A.; Pohl, N. L.* Fluorous-Based Carbohydrate Microarrays. J. Am. Chem. Soc. 2005, 127, 13162-13163.

• Zea, C. J.; Pohl, N. L.* Unusual Sugar Nucleotide Recognition Elements of Mesophilic Versus Thermophilic Glycogen Synthases. Biopolymers, 2005, 79, 106-113.

• Dutca, L.-M.; Ko, K.-S.; Pohl, N. L.; Kostic, N. M.* Platinum(II) Complex as an Artificial Peptidase: Selective Cleavage of Peptides and a Protein by cis-[Pt(en)(H2O)2]2+ Ion under Different Irradiations. Inorg. Chem. 2005, 44, 5141-5146.

• Ko, K.-S.; Zea, C. J.; Pohl, N. L.* Strategies for the Chemoenzymatic Synthesis of Deoxysugar Nucleotides: Substrate Binding Versus Catalysis. J. Org. Chem. 2005, 70, 1919-1921.

• Pohl, N. L.* Functional Proteomics for the Discovery of Carbohydrate-Related Enzyme Activities. Curr. Opin. Chem. Biol. 2005, 9, 76-81. (invited review)

• Mizanur, R. M.; Jaipuri, F. A.; Pohl, N. L.* One-Step Synthesis of Labeled Sugar Nucleotides for Protein O-GlcNAc Modification Studies by Chemical Function Analysis of an Archaeal Protein. J. Am. Chem. Soc. 2005, 127, 836-837.

• Mizanur, R. M.; Zea, C. J.; Pohl, N. L.* Unusually Broad Substrate Tolerance of a Heat-Stable Archaeal Sugar Nucleotidyltransferase for the Synthesis of Sugar Nucleotides. J. Am. Chem. Soc. 2004, 126, 15993-15998.

• Ko, K.-S.; Zea, C. J.; Pohl, N. L.* Surprising Bacterial Nucleotidyltransferase Selectivity in the Conversion of Carbaglucose-1-phosphate. J. Am. Chem. Soc. 2004, 126, 13188-13189.

• Pohl, N.* Cellular Addresses: Step One in Creating a Glycocode. Chem. Biol. 2004, 11, 891-892. (invited commentary)

• Yu, Y.; Ko, K.-S.; Zea, C. J.; Pohl, N. L.* Discovery of the Chemical Function of Glycosidases: Design, Synthesis, and Evaluation of Mass-differentiated Carbohydrate Libraries. Org. Lett. 2004, 6, 2031-2033.

• Zea, C. J.; Pohl, N. L.* General Assay for Sugar Nucleotidyltransferases Using Electrospray Ionization Mass Spectrometry. Anal. Biochem. 2004, 328, 196-202.

• Jaipuri, F. A.; Jofre, M. F.; Schwarz, K.; Pohl, N. L.* Microwave-assisted cleavage of Weinreb amide for carboxylate protection in the synthesis of a (R)-3-hydroxyalkanoic acid. Tetrahedron Lett. 2004, 45, 4149-4152.

• Zea, C. J.; Pohl, N. L.* Kinetic and Substrate Binding Analysis of Phosphorylase b Via Electrospray Ionization Mass Spectrometry: A Model for Chemical Proteomics of Sugar Phosphorylases. Anal. Biochem. 2004, 327, 107-113.

• Zea, C. J.; MacDonell, S. W.; Pohl, N. L.* Discovery of the Archaeal Chemical Link Between Glycogen (Starch) Synthase Families Using a New Mass Spectrometry Assay. J. Am. Chem. Soc. 2003, 125, 13666-13667.

• Jaipuri, F. A.; Bower, B. D.; Pohl, N. L.* Protic Acid-Catalyzed Polymerization of Beta-Lactones for the Synthesis of Chiral Polyesters. Tetrahedron: Asymmetry 2003, 14, 3249-3252.

• Ko, K.-S.; Kruse, J.; Pohl, N. L.* Synthesis of Isobutyl-C-galactoside (IBCG) as an Isopropylthiogalactoside (IPTG) Substitute for Increased Induction of Protein Expression. Org. Lett. 2003, 5, 1781-1783.

• Pohl, N. L.* Nonnatural Substrates for Polyketide Synthases and their Associated Modifying Enzymes. Curr. Opin. Chem. Biol. 2002, 6, 773-778. (invited review)

• Pohl, N.*; Clague. A.; Schwarz, K. Chiral Compounds and Green Chemistry in Undergraduate Organic Laboratories: Reduction of a Ketone By Sodium Borohydride And Baker’s Yeast. J. Chem. Educ. 2002, 79, 727-728.

• Pohl, N. L.* Developing New Antibiotics With Combinatorial Biosynthesis. J. Chem. Educ. 2000, 77, 1421-1423. (invited review)

Partial List of Publications via PubMed