A publication of the Association of Laboratory Technicians August 1999, Volume IX

Editor’s Desk: The Financial Planning Series continues through November. The schedule is on page 2. Thank you to Bruce Pesch, who suggested the series and made the necessary arran gements.

ALT is a sponsor for the first Immunology Workshop scheduled for October 1999 at Iowa State University. There is no charge and anyone may attend. The schedule is updated, so check details on page 2.

Other items in this issue include: a sneak preview of symposium 2000 speakers, and a reminder that the nominations committee will be looking for candidates for the 2000 ALT Board of Directors.

As always, we are open to suggestions for articles for Technically Speaking. If you have something you feel is news worthy, please send it to me.

W. Hambly, NADC, B9, 2300 Dayton Ave., Ames, IA 50010


We all extend a warm welcome TO ALT’S newest members:

Jeff Mason of the Zoonotic Diseases Research Unit of NADC, Ames, IA

John K. Roberts, Jr. of the Development Laboratory of Arizona Chemical Company in Panama City, FL. (People are visiting and using the website!)

Lori Dethloff currently employed by Boehringer Ingelheim Vetmedica, Ames, IA. On August 30th, Lori will move to the Zoonotic Disease Research Unit of NADC, Ames, IA.


August – November: Financial Planning Lunch & Learn Series– Investments, Estate Planning, & Retirement. See page 2 for a complete schedule and locations.

September- October: Nominations committee prepares the candidate list for the 2000 Board of Directors Ballot.

November General Meeting: meet the ALT board candidates

December: Elections, Register for symposium

February : Tuesday, February 1, 2000: ALT 9th annual Symposium, Gateway Center, Ames, IA

Board Meetings: September 7, October 5, November 2, December 7, and January 4, 2000. The Board meets at 8:15 am on the first Tuesday of the month. Meeting place to be announced.


Chad Reinke of the Zoonotic Diseases Research Unit at the National Animal Disease Center (NADC) is August’s featured technician.

In 1996, Chad received his BS in Animal Science at Iowa State University (ISU).

In the 1995, he became a Research Assistant for Dr. Ted Kramer at ISU Veterinary Medical Research Institute. While there, he studied in vitro selection of avirulent immunogenic Salmonella enteritis (SE) which contributed to the development of a live attenuated strain of SE suitable for vaccination of laying hens against fecal shedding and egg transmission of SE, one of the most common causes of food poisoning in people.

After two successful years, Chad accepted a Biological Laboratory Technician position with Dr. David Miller at the NVSL Diagnostic Bacteriology Laboratory. During his time there, he pe rformed isolation and identification of aerobic and anaerobic pathogenic bacteria from animal tissues and fluids.

In November 1998, he accepted a Biological Laboratory Technician position with Dr. Judy Stabel at the NADC in the Johne’s Disease Research Project. Current research is focused on the food safety issue of milk containing Mycobacteriu m paratuberculosis and its potential link to Crohn’s Disease in man.

His research interests include preventive medicine and zoonotic diseases of intracellular pathogens. Chad says, "I like the everyday challenge that research provides and the opportunity to enhance the health and safety of people a nd animals. I also enjoy hunting, fishing (unfortunately the secret place cannot be shared), boating, and spending time with family."

Chad has been a member of ALT since 1997. Currently, he is Vice-President of ALT and will become President at the February Symposium. One of the responsibilities of the ALT Vice-President is chairing the Membership Committee. Chad u pdated the list of volunteers who do postings for ALT. He was also instrumental in updating ALT’s 1999 brochure and in distributing information about ALT to prospective new members. He will soon be activating the Nominations Committee and recruiting boa rd candidates. Help him out and volunteer to be on the ballot!

In addition to enjoying the annual symposium, the quarterly newsletter, and special ALT-sponsored events, Chad enjoys the opportunity to meet numerous professionals in his field. He has made new friends and enjoys sharing ideas with th em.

Current Topics Seminars in Immunology Interdepartmental Immunobiology Program

Dr. Randy Sacco, NADC scientist and workshop Faculty Coordinator, has arranged an impressive lineup for the Mucosal Immunology Workshop this fall. Presentations are on Wednesday’s, October 6th, 20th, and 27 th, and November 3rd, and 11th, at 4:10 p.m. Locations vary, so check the listing below. There are no fees for these presentations and everyone is welcome.

Wednesday, October 6, 1999

Speaker: Suzanne Michalek, Department of Microbiology, University of Alabama at Birmingham

"The Mucosal Immune System; Novel Approaches for the Development of Vaccines against Mucosal Pathogens"

2532 Veterinary Medicine, 4:10 p.m.

Faculty Host: Michael Wannemuehler, VMPM

Wednesday, Oct 20, 1999

Speaker: Margaret Parr, Department of Anatomy, Southern Illinois University, School of Medicine

"Protective Immunity against HSV-2 in the Mouse Vagina"

2532 Veterinary Medicine, 4:10 p.m.

Faculty Host: Susan Carpenter, VMPM

Wednesday, October 27, 1999

Speaker: John Butler, Department of Microbiology, University of Iowa College of Medicine

"Colonization of the Newborn Piglet Selectively Results in Diversification of the Mucosal Antibody Repertoire"

2532 Veterinary Medicine, 4:10 p.m.

Faculty Co-Hosts: Louisa Tabatabai, BBMB and Ricardo Rosenbusch, VMPM

Wednesday, November 3, 1999

Speaker: Joseph Urban, Immunology and Disease Resistance Laboratory, USDA, Beltsville, MD

"IL-4a chain-Dependent Stat6 Activation Determines Resistance to Several Different Gastrointestinal Nematodes"

2532 Veterinary Medicine, 4:10 p.m.

Faculty Host: James Harp, NADC

Thursday, Nov 11, 1999

Speaker: Erika Crouch, Department of Pathology, Washington University School of Medicine

Collagenous C-type Lectins: Contributions to the Innate Host Defense against Inhaled Microorganisms"

2226 Veterinary Medicine, 4:10 p.m.

Faculty Host: Mark Ackermann, VPath

Sponsors of this workshop: An Sci, BBMB, FSHN, NADC NVSL, VCS, VDPAM, VMPM, VMRI, Zoo/Gen, Fort Dodge Labs, Association of Laboratory Technicians, Immunobiology Program, Miller Foundation of the Graduate College, and the College of Agri culture.

Financial Planning – Lunch & Learn Series continues. Reports are that attendance has been good at both NVSL and NADC. The audience is co mposed of both members and non-members and technicians to doctors. The informative sessions, presented by Karen Petersen, CFP, and Les Rousseau, CFP, of American Express Financial Services, will continue through November. "Investments" is the to pic in August and September, Retirement in October and Estate Planning in November. Presentations begin at noon. Times and locations are listed below. Note that the sessions at ISU have been canceled. If you miss a session at one location, you can cat ch it another week at the other location. There is no charge for sessions and everyone, both members and non-members, is welcome.




Investments -Types

Aug. 4

NADC auditorium


Aug. 11

NVSL west cafeteria

Investments – Strategies and Asset Mix

Sept. 8

NADC auditorium


Sept. 15

NVSL west cafeteria


Oct. 6

NADC auditorium


Oct. 13

NVSL west cafeteria

Estate Planning

Nov. 3

NADC auditorium


Nov. 10

NVSL west cafeteria


Vice President:
Past President:
Deb Owens
Chad Reinke
Wendy Hambly
Sam Humphrey
Brad Coates
Deb Hinrichsen
Bruce Pesch
Dawn Toms

Editorial Staff:

Editor: Wendy Hambly 515-239-8365

Board Liaison: Bruce Pesch 515-239-8626

Submission deadline for the November issue of Technically Speaking is October 15th, 1999. If you have suggestions for someone for the Featured Tech, have received a work-related award, or have information to share with ALT members, send it to me.

"Let’s Go Surfin’ Now, Everybody’s Surfin’ Now……."

If you haven’t visited ALT’s website for awhile, take a look. Keith Bidne is doing a great job. If you have some ideas or links to other scientific pages let Keith know.

The website address is : Congratulations! You are here!

Symposium 2000

Mark your calendars! The Ninth Annual ALT Symposium is taking shape. On February 1, 2000, at the Holiday Inn Gateway Center, Ames, IA, ALT will host its 9 th annual symposium. Vendors have been invited, and the board is awaiting their responses. The speaker slots are almost filled. Those who have agreed to speak include:

  • Dennis Byron of Pioneer Hybrid International
  • Dr. Randy Sacco, NADC Immunologist
  • Dr. Bruce Carter, former Iowa Veterinary-In-Charge
  • Dr. Naida Loskutoff, reproduction physiologist at the Henry Doorly Zoo, Omaha, NE

The board is waiting for confirmation from other speakers.

More detailed information and presentation titles will be available for the November issue of Technically Speaking.

TECH TIP: When harvesting cells from tissue for culturing, try this method. Mince tissues by rubbing tissue pieces between the frosted surfaces of 2 slides. It is fast and easier than forcing tissue through mesh.

Have a tip? Share it with the rest of us!

BE ACTIVE: Get involved in ALT and help ensure that ALT will continue to provide learning opportunities for technicians. The ALT symposium, Tech Week activities, and informative general meeting s happen because your ALT Board of Directors and activity chairpersons plan these activities.

The annual ALT symposium provides exposure to some excellent speakers. Although every presentation may not be directly related to what you are doing in the lab today, it maybe tomorrow. It is a chance to learn about new procedures or glean a better understanding a new technique or device. It also offers an opportunity to talk with numerous vendors about new products or problems your are having with current products or procedures, and to ask questions about new procedures you may be p lanning to try.

"Symposium Happens" does not apply. Your ALT board begins planning the next symposium as soon as the current year’s symposium is over. Reserving the facilities, securing speakers and vendors, and putting the packets togeth er are all done by the board.

As indicated earlier, the board is an elected body. ALT’s Vice-President, Chad Reinke, is setting the wheels of the election bandwagon in motion. He is forming the NOMINATIONS COMMITTEE, which prepares the ballot of candidates for t he election of the ALT Board of Directors. The current Vice-President moves to the President position and the remaining positions are filled by election. Candidates are needed for the positions of Vice-President, Secretary, Treasurer, and 3 Members-At-L arge.

ALT needs YOUR cooperation. ALT needs YOU to get involved. Although actual voting for the board is in November, Chad will form the nominations committee by September 1, 1999 to ensure plenty of time to provide members with a full ball ot for the Board of Directors. Anyone interested in being part of this committee, please phone Chad at 515-663-7487 or e-mail him

The second phase of "getting involved" is volunteering to be on the ballot. We need a full ballot – 2 or 3 individuals running for each of the offices of VP, Secretary, and Treasurer and 5 or 6 individuals running for the 3 At-Large positions. It is time FOR YOU TO get involved AND Ensure that ALT continues.

Just fill out the form below and return it to Chad Reinke, NADC A17, 2300 Dayton Ave., Ames, IA 50010 or contact him by phone 515-663-7487 or e-mail:

Yes, I would be willing to be on the ballot for the year 2000 ALT Board of Directors. Please consider me for the following position(s):

  • Vice-President
  • Secretary
  • Treasurer
  • Member-At-Large