Kamel Harrata, Ph.D.
Mass Spectrometry Laboratory, Iowa State University
Department of Chemistry
1707 Gilman Hall, Ames IA 50011
phone: (515) 294 5544
Current position Mass Spectrometry Supervisor
E-mail address kamel@iastate.edu
Web site http://www.public.iastate.edu/~kamel
Citizenship: USA

EDUCATION

-Post-Doctorate Research Associate (1990-1993) at the University of New Orleans, Dr. R. B. Cole group. "Electrospray fundamentals, and analysis of toxins from sea food and bacteria from cotton."

 -Ph.D.: Laboratoire de Chimie Organique Structurale, Mass Spectrometry Laboratory, University Pierre & Marie Curie, Paris France (1989). "Reactivite en phase gazeuse de cations radicaux: Ceto-esters, Dioxolannes-esters et de cation acylium substitues."

 -Master's degree in Spectrochemistry, University Pierre & Marie Curie Paris (1986). "Humic and Fulvic acids extraction and size exclusion chromatography analysis."
 

CURRENT OCCUPATION

 Mass spectrometry laboratory supervisor at the Chemical Instrumentation Facility- Chemistry Department, Iowa State University.
Duties and responsibilities include:

- Managing facility mass spectrometers and related equipment...
- Establish and document correct methods and procedures.
- Advise and consult with faculty and all ISU researchers ...
- Supervise the mass spec technician.
- Proposal preparation.
- Teaching graduate students how to use some of the MS lab equipements.

 RESEARCH

Current research:

* Extractions and trace analyses of active species in natural products such as herbal medecines.

* HPLC/MS/MS of glycoproteins.

 * HPLC/ESIMS analysis of caratenoids and digested proteins and polypeptides.

 * Development of capillary zone electrophoresis-electrospray mass spectrometry for protein analysis.

 * On-line Micellar Electrokinetic Chromatography-Electrospray Mass Spectrometry analysis of herbicide mixtures.

 * On-line Capillary Isoelectric Focusing (CIEF) Electrospray Mass Spectrometry (ESMS) for the separation of proteins according to their isoelectric point (pI) differences.

-(1991-1993 University of New Orleans) Post-Doctorate Research Associate:

 * Application of Liquid Chromatography-Electrospray Mass Spectrometry to the structural analysis of biological toxins (in Cotton and Seafood).
* Negative ion mode electrospray investigations: implications to the mechanism of ionization.
* Adaptation of a single quadrupole HP5985 mass spectrometer to the FAB ionization. This includes the design of the ion source and installation of a Cesium Gun.

-(1987-1990 Paris France) Ph.D.:

*Study of gas phase decompositions of bifunctional molecules such as: Keto-esters, Dioxolane-esters, and Keto-alcohols. Mass-analyzed Ion Kinetic Energy and Collisionally-Activated Dissociations mass spectra were recorded on VG ZAB-HSQ and Nermag R30-10 triple quadrupole. All the labeled products were synthesized along with corresponding compounds using D, 18O and 13C isotopes.

-(1986-1987 Paris France) Master's:

*Extraction and analysis of sedimentary organic matter. After extraction of marine sediment, Humic and Fulvic acids were analyzed by HPLC methodology.
 
 

INSTRUMENTATION EXPERTISE:

-Mass Spectrometry:

* Finnigan 4500 (single quad).
* Finnigan Magnum ITD GC-MS (ion trap).
* Finnigan TSQ 700 (triple quad).
* VG ZAB-HSQ (BEQQ configuration).
* Nermag R30-10 (triple quad).
* Hewlett-Packard 5985A (single quad).
* Kratos MS30 and MS50 (EB configuration).
* Vestec model 201 (Electrospray only).
* Bruker Proflex MALDI-TOF.
* Shimadzu LC-MS 2010.
* Micromass GC-TOF.

- Chromatography:

 * Beckman 340 Organizer.
* HP1090 Liquid Chromatograph.
* Rainin Rabbit-HP.
* Applied Biosystem 140A

PUBLICATIONS

 1. "On-line Micellar Electrokinetic Chromatography-Electrospray Ionization Mass Spectrometry using Anodically Migrating Micelles". Anal. Chem. 69,1820-1826 (1997).

 2. "Two-Dimensional Analysis of Recombinant E. Coli Proteins by Capillary Isoelectric Focusing Electrospray Ionization Mass Spectrometry". Anal. Chem. 69, 3177-3182 (1997).

 3. "High Resolution Capillary Isoelectric Focusing-Electrospray Ionization Mass Spectrometry For Hemoglobin Variants Analysis". Anal. Chem. 68, 2482-2487 (1996).

 4. "Capillary Isoelectric Focusing-Electrospray Ionization Mass Spectrometry for Transferin Glycoforms Analysis". Anal. Biochem. 243, 140-149 (1996).

 5. "Comparison of Protein Separation in Capillary Zone Electrophoresis and Capillary Isoelectric Focusing Interfacing with Electrospray Mass Spectrometry". J. Mass Spectrom. 31, 1284-1290 (1996).

 6. "On-Line Partial Filling Micellar Electrokinetic Chromatography-Electrospray Ionization Mass Spectrometry". J. Chromatogr. A 749, 219-226 (1996).

 7. "Capillary Isoelectric Focusing-Electrospray Mass Spectrometry For Protein Analysis".
Anal. Chem. 67, 3515-3519 (1995).

 8. "Modern Mass Spectrometry for Structural Analysis of Endotoxins".
Proceedings Beltwide Cotton Conferences, vol. 1, 310-313 (1993).

 9. "Solvent Effect on Analyte Charge State, Signal Intensity, and Stability in Negative Ion Electrospray Mass Spectrometry; Implications for the Mechanism of Negative Ion Formation".
J. Mass Spectrom. 4, No 7, 547-556 (1993).

 10. "Electrospray Mass Spectrometry for Characterization of Lipid A from Enterobacter agglomerans".
Biological Mass Spectrometry, vol. 22, 59-67 (1993).

 11. "Charge State Distribution and Discharge Suppression in Negative Ion Electrospray Mass Spectrometry using Chlorinated Solvents".
Rapid Commun. Mass Spectrom., No 6, 536-539 (1992).

 12. "Implementation of Liquid Secondary Ion Mass Spectrometry on Quadrupole Mass Spectrometers: Strategy, Optimization, and Evaluation".
Analytica Chimica Acta, vol. 267, No.1, 121-129 (1992).

 13. "A collisional activation study of [C5H7O2]+ ions from Methyl-2 Methyl Dioxolane-2 Propanoate".
Advances in Mass Spectrometry,vol. 11B, p. 931 (1989).

 14. "Fragmentation of Acylium ions from Methyl Levulinate. Isomerization Processes Involving Carbon and Oxygen Atoms of Both Carbonyl Groups".
Organic Mass Spectrometry, vol. 24, 977-983 (1989).

PROFESSIONAL PRESENTATIONS

 1. "Capillary Isoelectric Focusing-Electrospray Ionization Mass Spectrometry for Transferrin Glycoforms Analysis". The Pittsburgh Conference, 1997.

2. "On-line Integration of Micellar Electrokinetic Chromatography with Electrospray Ionization Mass Spectrometry. The Pittsburgh Conference, Atlanta, 1997.

3. "On-Line Capillary Isoelectric Focusing-Electrospray Mass Spectrometry Analysis of Proteins". The Pittsburgh Conference, Chicago, 513P, 1996.

4. "Nouvelle Methode de Synthese des 2-Methyl (2-Phenyl) Benzimidazoles a patir du 2-Methylmercapto-4-Phenyl-1,5-Benzodiazepine". 2eme Rencontre Nationale sur la Chimie Heterocycliques (R.N.C.H2), 3-4 Mai 1996, Marrakech, Morocco.

5. "Synthese de Nouvelles Molecules de Type 1,2-Bis-[Benzimidazol-2-YL] Ethane". 2eme Rencontre Nationale sur la Chimie Heterocycliques (R.N.C.H2), 3-4 Mai 1996, Marrakech, Morocco.

 6. "Capillary Isoelectric focusing-Electrospray Mass Spectrometry for Protein Characterization".
17th International Symposium on Capillary Chromatography and Electrophoresis, May 9, 1995

 7. "Fragmentation Behavior of Lipid A's in Electrospray vs. Plasma Desorption Mass Spectrometry".
40th Annual Conference on Mass Spectrometry and Allied Topics, Washington DC, June 2, 1992.

 8. "Solvent Effects in Negative Ion Electrospray Mass Spectrometry".
40th Annual Conference on Mass Spectrometry and Allied Topics, Washington DC, June 2, 1992.

 9. "Structural Determination of Endotoxins by Electrospray Mass Spectrometry".
Pittsburgh Conference & Exposition on Analytical Chemistry and Allied Spectroscopy, New Orleans, LA, March 9, 1992.

 10. "Transformation of a GC-MS Instrument to Perform Liquid SIMS".
12th International Mass Spectrometry Conference, Amsterdam, The Nederlands, August 26-30, 1991.

 11. "Electrospray Mass Spectrometry of Lipid A from Enterobacter agglomerans". 12th International Mass Spectrometry Conference, Amsterdam, The Nederlands, August 26-30, 1991.

 12. "Liquid Secondary Ion Mass Spectrometry of Biomolecules". 21st Annual Symposium Advances in Applied Analytical Chemistry, New Orleans, LA, May 22, 1991.

 13. "Modification of a Quadrupole Mass Spectrometer for Liquid SIMS".
39th Annual Conference on Mass Spectrometry and Allied Topics, Nashville, TN, May 20, 1991.

 14. "Mise en evidence par marquage a 13C et 18O des reactions d'isomerisation qui accompagnent l'elimination de CO dans les ions [M-CH3]+ et [M-OCH3]+ du Levulinate de methyle".
7eme Congres de Spectrometry de Masse, Rennes, 1989.
 

15. "Structural Determination of [C5H7O2]+ ions from Methyl-2 Methyl Dioxolane-2 Propanoate using MS/MS techniques". International Mass Spectrometry Conference (Bordeaux) Aug 29-Sep 2, 1988.
 

EDUCATIONAL ACTIVITIES

 - Training graduate students and post-doctorates for routine use of both Finnigan machines, Magnum ITD and TSQ700.

 - Organization of mass spectrometry workshops at the university.

 - Teaching a one session class entitled "The Tools of Mass Spectrometry".

 - Ames section chair of the American Chemical Society for the year 1996.

MEMBERSHIP:

 - American Society for Mass Spectrometry.
- American Chemical Society.
- Societe Francaise de Chimie.

Computers: Familiar with DOS, Windows, Macintosh, and Unix (Ultrix) systems.

REFERENCES:

 - Metzler David E, Distg Prof.
Iowa State University, 4106 Molecular Biology, Ames Iowa 50011.
phone: (515) 294 2828

 - Professor Cheng Lee
University of Maryland
College Park, MD 20742
phone: (301) 405 1020

- Professor Valerie Sheares
Iowa State University, Department of Chemistry, Ames IA 50011
phone: (515) 294 2474

- Robert Angelici, Distg Prof.
Iowa State University, Department of Chemistry, Ames IA 50011.
phone: (515) 294 2603