FILARIN, A NOVEL INVERTEBRATE INTERMEDIATE FILAMENT PROTEIN PRESENT IN AXONS AND PERIKARYA OF DEVELOPING AND MATURE LEECH NEURONS

 

 

Kristen M. Johansen and Jørgen Johansen

 

 

ABSTRACT

 

The lan 3-8 monoclonal antibody recognizes a 63 kDa antigen which is associated with the cytoskeleton in leech neurons (McKay et al., 1984, J. Comp. Neurol. 226:448-455). We have used this antibody to clone a novel invertebrate neuronal intermediate filament protein, filarin, by screening an expression vector library. A full length clone of 2.2 kb identified by the antibody was isolated and sequenced. The protein contains a coiled-coil rod domain typical of the superfamily of intermediate filament proteins flanked by unique N and C-terminal domains. The highest homology of filarin is to the alternatively spliced squid brain intermediate filament protein (Szaro et al., 1991, J. Biol. Chem. 266:15035-15041.), the only other invertebrate neuronal intermediate filament in the data bank. However, apart from extensive homology in the two end regions of the rod domain the similarity of the two proteins is limited to the general coiled-coil structure of intermediate filaments. Thus filarin may represent a novel type of invertebrate neuronal intermediate filament protein. Filarin contains the extra 6 heptads characteristic of lamins and of all cytoplasmic invertebrate intermediate filaments analyzed so far. By Northern analysis it appears that filarin is not alternatively spliced since only a single transcript of 2.2 kb is recognized by the clone. Using the lan 3-8 antibody to follow its developmental expression, we found that filarin is present in all known neurons in the central and peripheral nervous system.

 

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