John E. Mayfield
3306 Buchanan Ct.
Ames IA, 50010
(515) 232 8862
Department of Genetics, Development, and Cell Biology
Iowa State University
Ames, Iowa 50011
2011-present Professor emeritus
1989-2011 Professor, Iowa State University
1995-2004 Associate Dean of the Graduate College, Iowa State University
1994-1995 Chair, Interdepartmental Genetics Major
1983-1986 Chair, Molecular, Cellular and Developmental Biology Interdepartmental Graduate Major
1981 Visiting Lecturer, Trinity College, University of Dublin, Dublin, Ireland
1979-1989 Associate Professor of Zoology, Iowa State University
1977-1979 Assistant Professor of Zoology, Iowa State University, Ames, Iowa
1977 Visiting Associate, Harvard University Biological Laboratories
1971-1977 Assistant Professor of Biology, Carnegie-Mellon University
1971 Instructor of Developmental Biology, California Institute of Technology
1968-1971 Postdoctoral Fellow, California Institute Technology
1968 University of Pittsburgh, Pittsburgh, PA Ph.D. Biophysics
1965 University of Pittsburgh, Pittsburgh, PA M.S. Biophysics
1963 College of Wooster, Wooster, Ohio B.S. Physics
2005-2006 President of the Midwestern Association of Graduate Schools
2007 Institutional Service Award, College of Liberal Arts and Sciences
Taught thousands of students for 40 years at both the graduate and undergraduate level. Subjects included Cell and Molecular Biology, Introductory Biology, and Complex Adaptive Systems.
John E. Mayfield (2013). The Engine of Complexity, Evolution as Computation. Columbia University Press.
John E. Mayfield (1965). Ultraviolet Dichroism of Flow Oriented Bacteriophage fd. Masters Thesis, University of Pittsburgh.
John E. Mayfield (1968). Quantitative Interpretation of Ultraviolet Flow Dichroism. Ph.D. Thesis, University of Pittsburgh.
c) Peer Reviewed Scientific Articles
1 Irwin J. Bendet and John E. Mayfield (1967). "Ultraviolet Dichroism of fd Bacteriophage." Biophysical Journal 7, 111-119.
2 John E. Mayfield and Irwin J. Bendet (1970). "Quantitative Flow Dichroism. I. Correction for Disorientation in a Solution of Rods." Biopolymers 9, 655-668.
3 John E. Mayfield and Irwin J. Bendet (1970). "Quantitative Flow Dichroism. II. Form Dichroism at Ultraviolet Wavelengths." Biopolymers 9, 669-675.
4 John E. Mayfield and James Bonner (1971). "Tissue Differences in Rat Chromosomal RNA." Proc. Nat. Acad. Sci. 68, 2652-2655.
5 John E. Mayfield and James Bonner (1972). "A Partial Sequence of Nuclear Events in Regenerating Rat Liver." Proc. Nat. Acad. Sci. 69, 7-10.
6 David S. Holmes, John E. Mayfield, Gernot Sander and James Bonner (1972). "Chromosomal RNA: Its properties." Science 177, 72-74.
7 David S. Holmes, John E. Mayfield and James Bonner (1974). "Sequence Composition of Rat Ascites Chromosomal Ribonucleic Acid." Biochemistry 13, 849-855.
8 John E. Mayfield and John R. Ellison (1975). "The Organization of Interphase Chromatin in Drosophilidae. The Self Adhesion of Chromatin Containing the Same DNA Sequences." Chromosoma 52, 37-48.
9 John E. Mayfield and John E. Ellison (1975). "An Improved Method for Isolating DNA from Adult Drosophila." Drosophila Information Service 51, 144.
10 John E. Mayfield and John R. Ellison (1976). "The Fluoresence Localization of Mouse Satellite DNA in Interphase Nuclei." Chromosoma (Berl.) 54, 27-31.
11 R. Marino, K.N. Saksena, M. Schuler, J.E. Mayfield and P.A. Lemke (1976). "Double-Stranded Ribonucleic Acid in Agaricus bisporus." Applied and Environmental Microbiology 31, 433-438.
12 John E. Mayfield (1977). "A Comparison of the Differential Melting Profiles with the CsCl Density Profiles of DNA from Escherichia coli, Cow, Mouse, Rat, and Chicken." Biochem. Biophys. Acta 477, 97-101.
13 John E. Mayfield and Julie F. McKenna (1978). "A-T Rich Sequences in Vertebrate DNA, A Possible Explanation of Q-Banding in Metaphase Chromosomes." Chromosoma 67, 157-163.
John E. Mayfield, L.A. Serunian, L.M. Silver, and
S.C.R. Elgin (1978).
"A Protein Released by DNAase 1 Digestion of Drosophila Nuclei is Preferentially Associated with Puffs." Cell 14, 539-544.
15 John E. Mayfield, Julie F. McKenna, and Bruce Lessa (1980). "The Sequence Organization of Bovine DNA." Chromosoma 76, 277-294.
16 James A. Bassuk and John E. Mayfield (1982). "Major High Mobility Group Like Proteins of Drosophila melanogaster Embryonic Nuclei." Biochemistry 21, 1024-1027.
17 John E. Mayfield, Peter Good, Holly J. VanOort, Alphonso R. Campbell and David E. Reed (1983). "The cloning and cleavage site mapping of the DNA from Bovine Herpesvirus-1 (Cooper Strain)." Journal of Virology 47, 257-264.
18 Katherine Richardson, Renae M. Crosby, Peter Good, Nancy Rosen, and John Mayfield (1986). "Bovine DNA Contains a Single Major Family of Interspersed Repetitive Sequences." Eur. J. Biochem. 154, 349-358.
19 D. L. Rock, S. L. Beam, and J. E. Mayfield (1987). "Mapping Bovine Herpesvirus Type 1 Latency-Related RNA in Trigeminal Ganglia of Latently Infected Rabbits." Journal of Virology 61, 3827-3831.
20 John E. Mayfield, Betsy J. Bricker, Holly Godfrey, Renae M. Crosby, David J. Knight, Shirley M. Halling, Doris Balinsky and Louisa B. Tabatabai (1988). "The cloning, expression, and nucleotide sequence of a gene coding for an immunogenic Brucella abortus protein." Gene 63, 1-9.
21 Betsy J. Bricker, Louisa B. Tabatabai, Billy L. Deyoe, and John E. Mayfield (1989). "Conservation of antigenicity in a 31 kD Brucella Protein." Vet. Micro. 18, 313-325.
22 G. L. Lindberg, B. B. Shank, M. F. Rothschild, J. E. Mayfield, A. E. Freeman, C. M. Koehler, and D. C. Beitz (1989). "Characteristics of mammary mitochondria in lines of mice genetically divergent for milk production." J. Dairy Sci. 72, 1175-1181.
23 George W. Pugh, Jr., Louisa B. Tabatabai, Betsy J. Bricker, John E. Mayfield, Marshall Phillips, Timothy J. McDonald, and Emilie S. Zehr (1989). "Identification of a possible virulence factor for Brucella abortus infection in BALB/c mice." Am. J. Vet. Research, 50, 887-892.
24 D. R. Brown, C. M. Koehler, G. L. Lindberg, A. E. Freeman, J. E. Mayfield, A. M. Myers, M. M. Schutz and D. C. Beitz (1989) "Molecular Analysis of Cytoplasmic Genetic Variation in Holstein Cows." J. Animal Sci. 67, 1926-1932.
25 John E. Mayfield, John A. Bantle, Darla R. Ewalt, Vincent P. Meador, and Louisa B. Tabatabai (1990). "Detection of Brucella cells and cell components." In: Animal Brucellosis, K. Nielsen and J. R. Duncan, eds., CRC Press, Inc., Boca Raton, Fla., pp. 97-120.
26 Bonnie L. Beck, Louisa B. Tabatabai, and John Mayfield (1990). "A Protein Isolated from Brucella abortus is a Cu-Zn Superoxide Dimutase." Biochemistry 29, 372-376.
27 Thomas J. Stabel, John E. Mayfield, Louisa B. Tabatabai and Michael J. Wannemuehler (1990). “Oral Immunization of Mice with Attenuated Salmonella typhimurium Containing a Recombinant Plasmid which Codes for Production of a 31-kDa Protein of Brucella abortus.” Infect. Immun. 58, 2048-2055.
28 George W. Pugh, Jr., Louisa B. Tabatabai, Betsy J. Bricker, John E. Mayfield, Marshall Phillips, Emilie Zehr, and Carol Belzer (1990). "Immunogenicity of Brucella-extracted and recombinant protein vaccines in CD-1 and BALB/c mice." Am. J. Vet. Research 51, 1413-1420.
29 Henderson, L. M., Katz, J. B., Erickson, G. A., and Mayfield, J. E. (1990) “In vivo and In vitro Genetic Recombination Between Conventional and Gene-Deleted Vaccine Strains of Pseudorabies Virus”. Am. J. Vet. Res. 51, 1656-1662.
30 Betsy J. Bricker, Louisa B. Tabatabai, Bradley A. Judge, Billy L. Deyoe, and John E. Mayfield. (1990). Cloning, expression, and occurrence of the Brucella Cu-Zn superoxide dismutase. Infect. Immun. 58, 2935-2939.
31 Betsy J. Bricker, Louisa B. Tabatabai, and John Mayfield. (1991). "IgG Binding by a macromolecule on the surface of Brucella abortus." Mol. Immunol. 28, 35-39.
32 Carla M.
Koehler, Gary L. Lindberg, Daniel R. Brown, Donald C. Beitz,
A. Eugene Freeman, John E. Mayfield and Alan M. Myers. "Replacement of Bovine Mitochondrial DNA by a Sequence Variant Within a Single Generation." (1991) Genetics 129:247-255.
33 Thomas Stabel, John E. Mayfield, Louisa B. Tabatabai, and Michael J. Wannemuehler (1991) "Swine Immunity to an Attenuated Salmonella typhimurium Containing a Recombinant Plasmid Which Codes for Production of a 31-Kilodalton Protein of Brucella abortus." Infect. Immun. 59: 2941-2947.
34 Shirley M. Halling, Philipe G. Detilleux, Fred M. Tatum, Bradley A. Judge, and John E. Mayfield (1991) "Deletion of the BCSP31 Gene of Brucella abortus by replacement." Infect. Immun. 59:3863-3868.
35 Gary L. Lindberg, Carla M. Koehler, John E. Mayfield, Alan M. Myers, and Donald C. Beitz. (1992) “Recovery of Mitochondrial DNA from Blood Leukocytes Using Detergent Lysis”. Biochem. Gen. 30:27-33.
36 M. M. Schutz, A. E. Freeman, D. C. Beitz, and J. E. Mayfield. (1992) “The importance of maternal lineage on milk yield traits of dairy cattle”. J. Dairy Sci. 75:1331-1341.
37 Dennis Gor and John E. Mayfield. (1992). “Cloning and nucleotide sequence of the Brucella abortus groE operon”. Bioch. Bioph. Acta. 1130:120-122.
38 Thomas J. Stabel, John E. Mayfield, David C. Morfitt, and Michael J. Wannemuehler. (1993). “Oral immunization of mice and swine with an attenuated Salmonella choleraesuis cya/crp containing a recombinant plasmid”. Infect. Immun. 61:610-618.
39 Thomas J. Stabel, Zhengyu Sha and John Mayfield. (1994). “Periplasmic location of Brucella abortus Cu/Zn Superoxide Dismutase”. Vet. Microbiol. 38:307-314.
40 Zhengyu Sha, Thomas J. Stabel, and John E. Mayfield. (1994). “Brucella abortus catalase is a periplasmic protein lacking a standard signal sequence". J. Bacteriol. 176:7375-7377.
41 John E. Mayfield and Mel Duvall. (1996). “Anomalous phylogenies based on bacterial catalase gene sequences”. J. Molecular Evolution 42:469-471.
42 Jeonga Kim and John E. Mayfield. (1997) “Brucella abortus arginase and ornithine cyclodeaminase genes are similar to Ti plasmid arginase and ornithine cyclodeaminase”. Biochimica et Biophysica Acta 1354:55-57.
43 Dan Ashlock and John E. Mayfield. (1998) “Acquisition of General Adaptive Features by Evolution”. Evolutionary Programming VII. VW. Porto, N. Saravanan, D. Waagen, and AE. Eiben eds. Springer, Berlin. pp. 75-84..
44 Jeonga Kim, Zeng yu Sha, and John E. Mayfield. (2000) “Regulation of Brucella abortus catalase”. Infect. Immun. 68:3861-3866.
45 Jeonga Kim and John Mayfield. (2000) “Identification of Brucella abortus OxyR and Its Role in Control of Catalase Expression”. J. Bacterol. 182: 5631-5633.
46 John E. Mayfield. (2006) “Evolution as Computation”. Evolutionary Theory”
47. John E. Mayfield. (2007) “Minimal History, a Theory of Plausible Explanation”. Complexity 12: 48-53.
d) Graduate Education Articles
1. “Supporting Interdisciplinary Initiatives, the Interdepartmental Program Climate and the IGERT Impact at Iowa State University” in the Proceedings of the 63rd Annual Meeting of the Midwestern Association of Graduate Schools (2007).
2. “Student Crisis Management,” in the Proceedings of the 61th Annual Meeting of the Midwestern Association of Graduate Schools (2005).
3. “The Best Outcome is a Successful Career,” in the Proceedings of the 61th Annual Meeting of the Midwestern Association of Graduate Schools (2005).
4. “Integrated Multi-institutional Graduate Programs: Opportunities and Challenges Presented by the Internet,” in the International Journal of Innovation in Higher Education (2004)
5. “The Impact of Policy on Interdisciplinary Graduate Education at Iowa State University,” in Recruiting and Training Future Scientists: How Policy Shapes the Mission of Graduate Education, Merrill Advanced Studies Center, University of Kansas (2003).
6. “The Synergy Between Graduate and Undergraduate Education,” in the Proceedings of the 52nd Annual Meeting of the Midwestern Association of Graduate Schools (1996).
1. Recombinant Brucella abortus Gene Expressing Immunogenic Protein. Number: 5,023,174. John E. Mayfield and Louisa B. Tabatabai. (1991).
2. Brucella abortus diagnostic Reagents. Number: 5,188,936. Louisa B. Tabatabai, John E. Mayfield, and Bonnie Beck. (1993).