Associated
Professor and Deputy Dean
Faculty of Chemical Technology
Kaunas University of Technology
Radvilenu pl. 19
LT-3028 Kaunas
Lithuania
Phone
+370 7 451499
Fax +370 7 451582
jolanta.liesiene@ctf.ktu.lt
Education
1979 Kaunas Polytechnic
Institute, Doctor of Science (Ph.D)
1969 Kaunas
Polytechnic Institute, Diploma Engineer
Senate of Kaunas
University of Technology
Board of the
Faculty of Chemical Technology
European
Federation of Biotechnology
Lithuanian
Chemical Society
Research
Interests
Cellulose
chemistry and physics
Synthesis of
packings for liquid chromatography of proteins
Liquid
chromatography of biopolymers
Controlled drug
delivery systems
Other
Information
IWISE
(International Women in Science and Technology) fellow
Teaching
Courses on
Analytical and Preparative Chromatography,
Chemistry and
Technology of Cosmetics
Synthesis of
Packing for Chromatography of Proteins
Our
group has developed new cellulosic semi-rigid matrix called Granocel with
regulated pore size accessible for molecules till MM=2000000. Based on this
matrix, we have synthesized anion-exchangers, cation-exchangers, sorbents for
hydrophobic interaction chromatography (HIC), metal chelating sorbents for
immobilised metal affinity chromatography (IMAC) with different ligand content.
In
co-operating with biotechnologists we work on preparative chromatography of
biological active materials, such as a-interferon, human growth hormone. Immobilized metal affinity
chromatography on Chelat-Granocel, of a different content of ligand, was
examined as a method for the purification of recombinant human growth hormone,
somatotropin. Human growth hormone was found to exhibit high affinity to
chelating support charged with Cu(II). One step of immobilized metal affinity
chromatography on Chelat-Granocel gives 80% purification of the
protein. It was shown that the protein retention depends highly on the ligand
density. By regulating sorbent ligand density the favorable desorption was
achieved.
Presently we are developing packings for high-pressure chromatography of proteins, by coating silica with hydrophilic polymers, such as cellulose and its derivatives. Porous silica gels have excellent mechanical properties, however, negatively charged silanol groups exposed on the surface of silica cause a strong, even irreversible, nonspecific sorption of proteins. Moreover, silica gels are not stable in alkaline conditions. One of the ways to avoid these disadvantages is coating silica with hydrophilic polymers. We are currently studying adsorption of cellulose derivatives on silica surface.
Application of
Cellulose Derivatives for Controlled Drug Release
Among the polymers used in controlled drug delivery
systems cellulose derivatives are most popular due to their biodegradability,
non-toxicity and low price. In our work water-soluble cellulose derivatives
with tertiary amine groups were synthesised and evaluated for controlled drug
delivery. Our current emphases in this project are studies of the effect of
amino groups on release profile of drugs. It was found that in the case of
acidic drug concomitantly with
usual mechanisms, such as drug diffusion, polymer erosion, ionic interaction
contributes to drug release rate. Pressing properties of the polymers are
evaluated as well.
Selected
Publications
K.Racaityte, J.Liesiene, K.Radzevicius.
Anion exchange chromatography of proteins. Effect of charged groups of
stationary phase on protein binding. J.Liquid Chromatography & Related
Technics, 2001, in press.
J.Liesiene, K.Racaityte, M.Morkeviciene,
P.Valancius, V.Bumelis. Immobilized Metal Affinity Chromatography of Human
Growth Hormone: Effect of Ligand Density. Journal of Chromatography A, 764 (1997) 27-33.
A.Maruska, J.Liesiene, A.Serys. Regulation
of cellulose-based adsorbent granule morphology. Journal of Chromatography
A, 746, .147-160 (1996).
A.Serys, J.Liesiene, J.Urbonaviciene,
A.Maruska, K.Radzevicius, D.Valatkeviciute, V.Bumelis. Preparation and
characterization of cellulose based adsorbents for large scale hydrophobic
interaction chromatography. Journal of Liquid Chromatography, 17, 748-760 (1994).
A.Maruska, A.Serys, J.Liesiene,
J.Urbonaviciene, A.Zygas. Evaluation of morphological structure of packings by
gel permeation chromatography. Journal of Chromatography, 596, 157-164 (1992).
A.Maruska, J.Liesiene, A.Serys. The method for preparing granulated
cellulose. Lithuanian patent, Nr.2299 (1993).