·
Experienced with Light
and Electron Microscopy (TEM, SEM) and Confocal Scanning Laser Microscopy and
whole preparation techniques
of them.
·
Experienced to operate
of Light and Electron microscopes (STEM, SEM) , Confocal Scanning Laser
Microscope and X-ray diffraction.
·
Fluoresence in situ hybridization (FISH) techniques
(workshop at Texas A&M
University).
·
Introduction to
Molecular Biology Techniques workshop (one semester cource at Iowa
State University). Workshop in basic molecular biology techniques and
related procedures.
A. DNA Techniques.
Includes genetic engineering procedures, sequencing, PCR, and genotyping.
B. Plant Transformation.
Includes Agrobacterium and particle gun transformation, and analysis of
transformants (enzyme assay, PCR, Southern blot).
·
Experienced
regarding prepared variety of specimens (animal, botanical, microbiological, and
material) for all aspects of microscopy including brightfield, darkfield,
polarized, fluorescent, Nomarski, and phase contrast light microscopy, scanning
electron microscopy (SEM), and transmission electron microscopy
(TEM).
·
Performed
all techniques for microscopic evaluation including standard and microwave
processing procedures, paraffin sectioning, resin sectioning (both semi-thin and
thin ultramicrotomy with diamond knife for TEM grids), cryomicrotomy, staining
of slides for regular histochemistry, specialized
histochemistry, immunohistochemistry,
and in-situ hybridization, staining of sections on grids for TEM evaluation
using various heavy metals, staining grids for immunocytochemical and
histochemical TEM evaluation, positive and negative staining for microbiological
preparations, preparing carbon replicas for TEM evaluation, and preparing
and sputter coating critical point
dried samples for SEM evaluation.
·
Consulted
and assisted with design of new projects for researchers from university related
departments as well as private institutions/corporations for either proprietary
research or publications.
·
Operated
all specialized equipment including Leitz Orthoplan and Olympus SZH10 light
microscopes, JEOL 1200EX scanning/transmission electron microscope, JEOL 5800LV
scanning electron microscope, Kevex image analysis/X-ray microanalysis equipment
and software, Reichert Ultracut S ultramicrotome, A/O rotary microtome, Ted
Pella EM microwave processor and other ancillary
equipment.
·
Computer
Skills: Basic HTML (to make homepage), AdobePhotoshop and PageMaker,
Microsoft Office (word, excel. Powerpoint).