;nccneven.mh ;N-C', Ca-N dipolar correlation for psi torsion angle measurement. ;13C DQ excitation by CMR7, 15N-13C recoupling, 13C SQ reconversion ;Feng X et al. J. Am. Chem. Soc., volume 119, 12006, 1997 ;Costa PR et al. Chem. Phys. Lett., 280, 95, 1997 ;p3: 1H 90 pulse ;p15: contact pulse ;p4: 13C 360 pulse under low power pl11 ;p5: 13C REDOR 180 ;p1: 13C hard 90 ;p12: 15N hard 180 ;pl2: 1H power during CP ;pl3: 1H CP power ;pl4: 15N REDOR power ;pl11: 13C low power =7*wr ;pl10: 13C hard pulse power ;pl12: 1H power for decoupling ;pl22: 1H dec during CMR7 & REDOR ;d12: CMR7 phase switching time ;l0: 7, number of c7 elements in tau(exc). ;l2: REDOR loop,even, tmix=l2*tr "d3=2s/l31-p1-2u" "l1=aq/(p8*2)" "p4=1s/(l31*7)-d12" ;CMR7 360 pulse "p2=0.5s/(l31*7)-d12" ;CMR7 180 pulse "d25=0.25s/l31" ;REDOR "d26=0.25s/l31-p12/2" ;REDOR "d27=0.25s/l31-p5/2" ;REDOR "d28=0.25s/l31-p5-0.5u" "d2=0.5s/l31" 1 ze 2 d1 10u pl1:f1 10u pl2:f2 ;set decoupler power,1H 10u pl4:f3 2u:f1 ph2 ;preprare 13C phase for CP 2u:f2 ph1 1m rpp7 ;13C DQ phases 1m rpp9 1m rpp5 ;15N REDOR pulses 3 p3:f2 ph1 ;90 degree 1H pulse 0.5u pl3:f2 ;1H power for CP (p15 ph2):f1 (p15 ph3):f2 ;cross-polarization 1u cw:f2 pl12:f2 pl10:f1 ;----------- homo DQ excitation ------------------------ 4 1u:f1 ph4 p1:f1 ph4 ;flip to z hard 90; d3 do:f2 ;z-filter 1u cw:f2 pl22:f2 pl11:f1 5 d12:f1 ph7 (p4 ph7^):f1 ;R d12:f1 ph9 (p4 ph9^):f1 d12:f1 ph7 (p2 ph7^):f1 d12:f1 ph9 (p4 ph9^):f1 d12:f1 ph7 (p2 ph7^):f1 lo to 5 times l0 ;l0=7 equal to 4tr ;----------- NC REDOR ------------------------ d25 pl10:f1 ;tr/4 7 d26:f3 ph5 p12:f3 ph5^ d26 ;tr/2 per loop lo to 7 times l2 ;l2 = an even number d27:f1 ph21 ;tr/4 d2 p5:f1 ph21 ;13C 180 deg. pulse d2 d27 9 d26:f3 ph5 p12:f3 ph5^ d26 ;tr/2 per loop lo to 9 times l2 ;l2 = an even number d28:f1 ph22 ;tr/4 p5:f1 ph22 0.5u pl11:f1 ;----------- homo DQ reconversion ------------------------ 11 d12:f1 ph8 ;DQ recovert. (p4 ph8^):f1 ;R+90*n d12:f1 ph10 (p4 ph10^):f1 d12:f1 ph8 (p2 ph8^):f1 d12:f1 ph10 (p4 ph10^):f1 d12:f1 ph8 (p2 ph8^):f1 lo to 11 times l0 ;l0=7 equals 4tr 12 d3 do:f2 ;z-filter 1u cw:f2 pl12:f2 pl10:f1 1u:f1 ph6 p1:f1 ph6 ;flip to xy plane 1u:f1 ph0:r ;dead time, reset RF phase to detect 1u adc ph31 15 p8:f2 ph13 p8:f2 ph14 lo to 15 times l1 aq do:f2 1m ip8 ;increment by 90 degrees 1m ip10 rcyc=2 ;do NS scans 100m wr #0 exit ph0= 0 ph1= +x +x +x +x -x -x -x -x ;1H excitation ph3= +y ;1H spin lock ph2= +x +x +x +x +x +x +x +x ;13C CP +y +y +y +y +y +y +y +y -x -x -x -x -x -x -x -x -y -y -y -y -y -y -y -y ph4= +y +y +y +y +y +y +y +y ;13C storage -x -x -x -x -x -x -x -x -y -y -y -y -y -y -y -y +x +x +x +x +x +x +x +x ph6= +y -x -y +x +y -x -y +x ;13C read-back -x -y +x +y -x -y +x +y -y +x +y -x -y +x +y -x +x +y -x -y +x +y -x -y ph7= (56) 0 8 8 16 24 24 32 40 40 48 0 0 8 16 16 24 32 32 40 48 48 28 36 36 44 52 52 4 12 12 20 28 28 36 44 44 52 4 4 12 20 20 ph9= (56) 28 36 44 52 4 12 20 28 36 44 52 4 12 20 0 8 16 24 32 40 48 0 8 16 24 32 40 48 ph8= ph7 ph10= ph9 ph5= +x +y +x +y +y +x +y +x ;15N REDOR pulses -x -y -x -y -y -x -y -x ph21= +x +x +x +x -x -x -x -x +y +y +y +y -y -y -y -y ph22= -x -x -x -x +x +x +x +x -y -y -y -y +y +y +y +y ph13= (360) 10 ph14= (360) 0 ph31= 2 1 0 3 0 3 2 1 3 2 1 0 1 0 3 2 0 3 2 1 2 1 0 3 1 0 3 2 3 2 1 0