Publications
Hill, J.H., A.H. Epstein, J.R. Seibel, and W.A. Miller. 1994. Causal agent of rose rosette disease. Proceedings of the International Symposium: Rose Rosette and Other Eriophyid Mite-Transmitted Plant Disease Agents of Uncertain Etiology. Iowa State University.
Seibel, J.R., C.T. Lum, D.E.R. Sutherland, R.J. Howard, H.H. Balfour, and J.S. Najarian. 1980. The effect of ALG and cortico-steroids on adenosine deaminase activity in spleen cells from mice with and without previous Cytomegalovirus infection. Surg. Forum 31:54-57.
Lum, C.T., J.S. Najarian, D.E.R. Sutherland, J.R. Seibel, H.H. Balfour, W.G. Yasmineh, and R.J. Howard. 1979. ADA activity in spleen cells from mice injected with Cytomegalovirus. Surg. Forum 30:276-279.
.Seibel, J.R., and L.H. Fuchigami. 1978. The relationship between vegetative maturity and the onset of winter dormancy in red-osier dogwood. J. Amer. Soc. Hort. Sci. 103:737-739.
Seibel, J.R., and L.H. Fuchigami. 1978. Ethylene production as an indicator of seasonal development in red-osier dogwood. J. Amer. Soc. Hort. Sci. 103:739-741.

RESEARCH ASSOCIATE I, February 1998 to October 1998
Department of Food Science and Human Nutrition, Iowa State University.
Supervisor: Dr. Clark F. Ford.
Part-time, temporary, hourly position.
My first objective was to develop an assay for the detection of thermostable mutants of the enzyme glucoamylase. This allowed us to screen nitrocellulose membrane-bound glucoamylase for thermostability, and we used the assay to screen PCR-generated mutants for this trait. I also spent a large portion of time working to bring the laboratory into compliance with OSHA and EPA requirements.

LABORATORY ASSOCIATE I, September 1997 to November 1997.
Department of Microbiology, Immunology, and Preventative Medicine, Iowa State University.
Supervisors: Drs. Charles O. Thoen and Ronald W. Griffith.
Part-time, temporary, hourly position.
I prepared the bacterial and fungal cultures and demonstrations for the pathogenic bacteriology laboratory class for Veterinary Medicine students.

ASSOCIATE SCIENTIST I, March 1996 through February 1997.
Department of Food Science and Human Nutrition, Iowa State University.
Supervisor: Dr. Earl G. Hammond.
All of my duties involved the identification and quantification of odorous compounds from pig manure. I used an ion-exchange column to obtain short-chain fatty acids which I then separated and quantified using gas chromatography. I also made decyl esters of these fatty acids and used a gas chromatograph-mass spectrometer (GC-MS) to verify their identities. In addition, I used GC-MS to measure other odorous compounds such as indole and cresols. It was my responsibility to prepare written reports and send them to the company funding the research.

RESEARCH ASSOCIATE I, July 14, 1995, to September 12, 1995.
Department of Food Science and Human Nutrition, Iowa State University.
Supervisor: Wei Wang Bidlack.
Part-time, temporary, hourly position.
I obtained high density lipoproteins from human blood using density gradient ultracentrifugation, tested different techniques for delipidation to obtain apolipoproteins, and checked the composition of the preparations using SDS-polyacrylamide gel electrophoresis.

RESEARCH ASSOCIATE I, July 1993 to June 30, 1995.
Department of Food Science and Human Nutrition, Iowa State University.
Supervisor: Dr. Clark F. Ford.
I worked in a genetic engineering laboratory primarily dedicated to the creation of new and improved versions of the enzyme glucoamylase. My projects involved expressing glucoamylase in yeast and in Aspergillus. I isolated plasmid DNA from yeast and E. coli, cut with restriction enzymes, separated fragments using agarose gel electrophoresis, ligated, electroporated into E. coli and yeast, and screened plasmid DNA using restriction enzyme analysis. I also transformed Aspergillus protoplasts. I produced glucoamylase in broth cultures of yeast and Aspergillus, performed assays for enzyme activity, purified glucoamylase using an affinity column, performed SDS-polyacrylamide gel electrophoresis of enzyme preps, and used thin-layer chromatography to detect the production of maltose and isomaltose from glucose. As laboratory manager, I was the Laboratory Safety Officer. I ordered supplies, hired and supervised hourly employees, and strove to maintain a pleasant and smooth-running laboratory.

RESEARCH ASSOCIATE I, October 1992 to July 1993.
Department of Zoology and Genetics, Iowa State University.
Supervisors: Drs. John Mayfield and Michael Wannemuehler.
I came on near the end of a project studying potential vaccines for brucellosis. My part of the project involved oral inoculation of mice with Salmonella expressing various levels of Brucella heat shock protein, then sacrificing mice at intervals to determine the levels of Salmonella in various tissues, set up spleen cell blastogenesis assays, and determine blood antibody levels using ELISAs. I also purified plasmid DNA from E. coli, cut out genes with restriction enzymes and ligated them into different plasmids.