Clark Ford's Publications

Publications based on Ph.D. Thesis:

   Ford, C., and W.-Y. Wang.  (1980).  Three new yellow loci in
        Chlamydomonas reinhardtii.  Mol. Gen. Genet. 179, 259-263.

   Ford, C., and W.-Y. Wang.  (1980).  Temperature-sensitive yellow
        mutants of Chlamydomonas reinhardtii.  Mol. Gen. Genet.  180, 5-10.

   Ford, C., S. Mitchell, and W.-Y. Wang.  (1981).  Protochlorophyllide
        photoconversion mutants of Chlamydomonas reinhardtii.  Mol. Gen.
        Genet. 184, 460-464.

   Ford, C., and W.-Y. Wang.  (1982).  Instability at the y-1 locus of
        Chlamydomonas reinhardtii.  Mol. Gen. Genet. 187, 286-290.

Publications co-authored with Postdoctoral Advisors:

   Ford, C., S. Mitchell, and W.-Y. Wang.  (1983).  Characterization of
        NADPH: Protochlorophyllide Oxidoreductase in the y-7 and  pc-1 y-7
        mutants of Chlamydomonas reinhardtii.  Mol. Gen. Genet. 192, 290-292.

   Ford, C., and M. M. Smith.  (1985).  Use of an oligonucleotide probe to
        detect transplacement of an amber mutation into a yeast histone H3
        gene.   Gene 37, 45-52.

Publications co-authored with Students and Collaborators:

   Sierks, M. R., C. Ford, P. J. Reilly, and B. Svensson.  (1989).  Site-
        directed mutagenesis at the active site Trp120 of Aspergillus awamori
        glucoamylase.  Protein Eng. 2, 621- 625.

   Sierks, M. R., C. Ford, P. J. Reilly, and B. Svensson.  (1990).  Catalytic
        mechanism of fungal glucoamylase as defeined by mutagenesis of
        Asp176, Glu179, and Glu180 in the enzyme from Aspergillus
        awamori.  Protein Eng. 3,: 193-198.

   Zhao, J., C. Ford, C. Glatz, M. Rougvie, and S. Gendel. (1990).
        Polyelectrolyte precipitation of ß-galactosidase fusions containing
        polyaspartic acid tails.  J. Biotechnol. 14, 273- 284.

   Evans, R., C. Ford, M. Sierks, Z. Nikolov, and B. Svensson.  (1990).
        Deletion analysis of the O-glycosylated region of Aspergillus
        glucoamylase: effect on activity and thermal stability. Gene 91, 131-134.

   Parker, D., C. Glatz, C. Ford, S. Gendel, M. Rougvie, and I. Suominen.
        (1990).  Recovery of a Charged-fusion protein from cell extracts by
        polyelectrlyte precipitation. Biotechnol. Bioeng. 36, 467-475.

   Chen, L., C. Ford, and Z. Nikolov.  (1991).  Adsorption to starch of a
        ß-galactosidase fusion protein containing the starch-binding domain from
        Aspergillus glucoamylase. Gene 99, 121-126.

   Chen, L., C. Ford, A. Kusnadi, and Z. Nikolov.  (1991).  Improved
        adsorption to starch of a ß-galactosidase fusion protein containing the
        starch-binding domain from Aspergillus glucoamylase.
        Biotechnol. Prog. 7, 225-229.

     Tzeng, T.-H., L. K. Lyngholm, C. Ford, and C. Bronson.  (1992).  A
        restriction fragment length polymorphism map and electrophoretic
        karyotype of the fungal maize pathogen Cochliobolus heterostrophus.
        Genetics 130, 81-96.

   Sierks, M., C. Ford, P. J. Reilly, and B. Svensson. (1993)  Fuinctional roles
        and subsite locations of Leu 177, Trp 178, and Asn 182 of Aspergillus
        awamori glucoamylase determined by site-directed mutagenesis.
        Protein  Eng. 6, 75-79.

   Suominen, I., C. Ford, D. Stachon, H. Heimo, M. Niederauer, H.
        Nurmeia, and C. Glatz. (1993) Enhanced recovery and purification of
        Aspergillus glucoamylase from Saccharomyces cerevisiae by addition
        of  poly(aspartic acid) tails.  Enzyme Microbiol. Technol. 15, 593-600.

    Kusnadi, A. R., C. Ford, and Z. L. Nikolov. (1993) Production of a
        functional starch- binding domain of Aspergillus glucoamylase I in
        Escherichia coli.  Gene 127, 193- 197.

   Bakir, U., P. M. Coutinho, P. A. Sullivan, C. Ford, and P. J. Reilly. (1993)
        Cassette mutagenesis of Aspergillus awamori glucoamylase near its
        general acid residue to probe its catalytic and pH properties.
        Protein Eng. 6, 939-946.

   Chen, H.-M., U. Bakir, P. J. Reilly, and C. Ford. (1994) Increased
        thermostability of Asn182 Ala mutant Aspergillus awamori
        glucoamylase.  Biotech. Bioeng. 43, 101- 105.

   Chen, H.-M., C. Ford, and P. J. Reilly.  (1994)  Substitution of asparagine
        residues in Aspergillus awamori glucoamylase by site-directed
        mutagenesis to eliminate N- glycosylateion and inactivation by
        deamidation.  Biochem. J. 301, 275-281.

   Niederauer, M. Q., I. Suominen, M. A. Rougvie, C. F. Ford, and C. E.
        Glatz.  (1994) Characterization and polyelectrolyte precipitation of
        ß-galactosidase containing genetic fusions of charged polypeptides.
        Biotechnol. Prog. 10, 237-245.

   Flory, N., M. Gorman, P. M. Coutinho, C. Ford, and P. J. Reilly.  (1994)
        Thermosensitive mutants of Aspergillus awamori glucoamylase by
        random mutagenesis: inactivation kinetics and structural interpretation.
        Protein Eng. 7, 1005-1012.

   Libby, C. B., C. A. G. Cornett, P. J. Reilly, and C. Ford.  (1994)  Effect of
        amino acid deletions in the O-glycosylated region of Aspergillus
        awamori glucoamylase.  Protein Eng. 7, 1109-1114.

   Chen, H.-M., C. Ford, and P. J. Reilly. (1995)  Identification and
        elimination by site- directed mutagenesis of thermolabile aspartyl bonds
        in Aspergillus awamori glucoamylase.  Protein Eng. 8, 575-582.

   Chen, L., P. Coutinho, Z. Nikolov, and C. Ford. (1995)  Deletion analysis
        of  the starch binding domain of Aspergillus glucoamylase.
        Protein Eng. 8, 1049-1055.

   Chen, H.-M., T. Panda, Y. Li, F. Bühler, C. Ford, and P. J. Reilly.  (1996)
        Effect of replacing helical glycine residues with alanines on the
        thermostability and production of Aspergillus awamori glucoamylase.
        Protein Eng. 9, 499-505.

    Li, Y., P. J. Reilly, and C. Ford.  (1997)  Effect of introducing proline residues
        on the stability of Aspergillus awamori glucoamylase.  Protein Eng. 10, 1199-1204.

    Fang, T.-Y.,  P. M. Coutinho, P. J. Reilly, and C. Ford.  (1998)  Mutations to
        alter Aspergillus awamori selectivity. I. Y48F, Y116W, Y175F, R241K, S411A
        and S411G.  Protein Eng 11, 119-126.

    Fang, T.-Y., P. M. Coutinho, P. J. Reilly, and C. Ford.  (1998) Mutations to
        alter Aspergillus awamori glucoamylase selectivity II. Mutation of residues 119
        and 121.  Protein Eng 11, 127-133.

    Fang, Tsuei-Yun and Clark Ford.  (1998)  Protein engineering of Aspergillus awamori
         glucoamylase to increase its pH optimum.  Protein Eng. 11, 383-388.

    Liu, Hsuan-Liang, Pedro M. Coutinho, Clark Ford, and Peter J. Reilly. (1998)
        Mutations to alter Aspergillus awamori glucoamylase selectivity III. N20C/A27C,
        A27P, S30P, K108R, K108M, G137A, 311-314 loop, Y312W and S436P.
        Protein Eng. 11, 383-398.

    Li, Yuxing, Pedro M. Coutinho, and Clark Ford. (1998) Effect on thermostability and
        catalytic activity of introducing disulfide bonds into Aspergillus awamori glucoamylase.
        Protein Eng. 11, 661-667.

    Allen, Martin J., Pedro M. Coutinho, and Clark F. Ford. (1998)  Stabilization of Aspergillus
        awamori glucoamylase by proline substitution and combining stabilizaing mutations.
        Protein Eng. 11, 783-788.

    Liu, Hsuan-Liang, Yann Doleyres, Pedro M. Coutinho, Clark Ford, and Peter J. Reilly (2000)
        Replacement and deletion mutations in the catalytic domain and belt region of Aspergillus
        awamori glucoamylase to enhance thermostability.   Protein Eng. 13, 655-659.

    Khan, Saber M., Peter J. Reilly, and Clark Ford (2000) Thermal and Molecular Characterization
        of Aspergillus awamori Glucoamylase Catalytic and Starch?Binding Domains.
        Starch/Stärke 52, 385-397

    Allen, Martin J., Erica B. Fuchs, and Clark F. Ford (2002).  Asp238®Asn creates a novel
        consensus N-glycosylation site in Aspergillus awamori glucoamylase.
        Starch/Stärke 54, 385-392.
 

Review Articles:

   Ford, C. F., I. Suominen, and C. E. Glatz.  (1991) Fusion tails for the
        recovery and purification of recombinant proteins.
        Protein Expres. Purif. 2, 95-107.

   Glatz, C. E., and C. F. Ford.  (1995)  Genetic Engineering to Enhance the
        Selectivity of Protein Separations.
        Appl. Biochem. Biotechnol. 54, 173-191.

   Ford, C.F. (1999)  Improving operating performance of glucoamylase by mutagenesis.
        Current Opinion in Biotechnology 10, 353-357.
 
 

Bioethics:

   Ford, C.  (1996)  The Ethics of Research.  Ethos (ISU) v. 42, p. 30.
        Reprinted in: Research and Graduate Education  (ISU) v. 8, No. 6, p. 1.

   Ford, C. (2000) Genetically Modified Foods: Curse or Blessing?  Sowel Review 13, 17-20.


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