Publications based on Ph.D. Thesis:
Ford, C., and W.-Y. Wang. (1980).
Three new yellow loci in
Chlamydomonas
reinhardtii. Mol. Gen. Genet. 179, 259-263.
Ford, C., and W.-Y. Wang. (1980).
Temperature-sensitive yellow
mutants of
Chlamydomonas
reinhardtii. Mol. Gen. Genet. 180, 5-10.
Ford, C., S. Mitchell, and W.-Y. Wang.
(1981). Protochlorophyllide
photoconversion
mutants of Chlamydomonas reinhardtii. Mol. Gen.
Genet. 184,
460-464.
Ford, C., and W.-Y. Wang. (1982).
Instability at the y-1 locus of
Chlamydomonas
reinhardtii. Mol. Gen. Genet. 187, 286-290.
Publications co-authored with Postdoctoral Advisors:
Ford, C., S. Mitchell, and W.-Y. Wang.
(1983). Characterization of
NADPH: Protochlorophyllide
Oxidoreductase in the y-7 and pc-1 y-7
mutants of
Chlamydomonas
reinhardtii. Mol. Gen. Genet. 192, 290-292.
Ford, C., and M. M. Smith. (1985).
Use of an oligonucleotide probe to
detect transplacement
of an amber mutation into a yeast histone H3
gene.
Gene 37, 45-52.
Publications co-authored with Students and Collaborators:
Sierks, M. R., C. Ford, P. J. Reilly, and
B. Svensson. (1989). Site-
directed mutagenesis
at the active site Trp120 of Aspergillus awamori
glucoamylase.
Protein Eng. 2, 621- 625.
Sierks, M. R., C. Ford, P. J. Reilly, and
B. Svensson. (1990). Catalytic
mechanism
of fungal glucoamylase as defeined by mutagenesis of
Asp176, Glu179,
and Glu180 in the enzyme from Aspergillus
awamori.
Protein Eng. 3,: 193-198.
Zhao, J., C. Ford, C. Glatz, M. Rougvie,
and S. Gendel. (1990).
Polyelectrolyte
precipitation of ß-galactosidase fusions containing
polyaspartic
acid tails. J. Biotechnol. 14, 273- 284.
Evans, R., C. Ford, M. Sierks, Z. Nikolov,
and B. Svensson. (1990).
Deletion analysis
of the O-glycosylated region of Aspergillus
glucoamylase:
effect on activity and thermal stability. Gene 91, 131-134.
Parker, D., C. Glatz, C. Ford, S. Gendel,
M. Rougvie, and I. Suominen.
(1990).
Recovery of a Charged-fusion protein from cell extracts by
polyelectrlyte
precipitation. Biotechnol. Bioeng. 36, 467-475.
Chen, L., C. Ford, and Z. Nikolov.
(1991). Adsorption to starch of a
ß-galactosidase
fusion protein containing the starch-binding domain from
Aspergillus
glucoamylase. Gene 99, 121-126.
Chen, L., C. Ford, A. Kusnadi, and Z. Nikolov.
(1991). Improved
adsorption
to starch of a ß-galactosidase fusion protein containing the
starch-binding
domain from Aspergillus glucoamylase.
Biotechnol.
Prog. 7, 225-229.
Tzeng, T.-H., L. K. Lyngholm,
C. Ford, and C. Bronson. (1992). A
restriction
fragment length polymorphism map and electrophoretic
karyotype
of the fungal maize pathogen Cochliobolus heterostrophus.
Genetics 130,
81-96.
Sierks, M., C. Ford, P. J. Reilly, and B.
Svensson. (1993) Fuinctional roles
and subsite
locations of Leu 177, Trp 178, and Asn 182 of Aspergillus
awamori
glucoamylase
determined by site-directed mutagenesis.
Protein
Eng. 6, 75-79.
Suominen, I., C. Ford, D. Stachon, H. Heimo,
M. Niederauer, H.
Nurmeia, and
C. Glatz. (1993) Enhanced recovery and purification of
Aspergillus
glucoamylase from Saccharomyces cerevisiae by addition
of poly(aspartic
acid) tails. Enzyme Microbiol. Technol. 15, 593-600.
Kusnadi, A. R., C. Ford, and Z. L.
Nikolov. (1993) Production of a
functional
starch- binding domain of Aspergillus glucoamylase I in
Escherichia
coli. Gene 127, 193- 197.
Bakir, U., P. M. Coutinho, P. A. Sullivan,
C. Ford, and P. J. Reilly. (1993)
Cassette mutagenesis
of Aspergillus awamori glucoamylase near its
general acid
residue to probe its catalytic and pH properties.
Protein Eng.
6, 939-946.
Chen, H.-M., U. Bakir, P. J. Reilly, and
C. Ford. (1994) Increased
thermostability
of Asn182 Ala mutant Aspergillus awamori
glucoamylase.
Biotech. Bioeng. 43, 101- 105.
Chen, H.-M., C. Ford, and P. J. Reilly.
(1994) Substitution of asparagine
residues in
Aspergillus
awamori glucoamylase by site-directed
mutagenesis
to eliminate N- glycosylateion and inactivation by
deamidation.
Biochem. J. 301, 275-281.
Niederauer, M. Q., I. Suominen, M. A. Rougvie,
C. F. Ford, and C. E.
Glatz.
(1994) Characterization and polyelectrolyte precipitation of
ß-galactosidase
containing genetic fusions of charged polypeptides.
Biotechnol.
Prog. 10, 237-245.
Flory, N., M. Gorman, P. M. Coutinho, C.
Ford, and P. J. Reilly. (1994)
Thermosensitive
mutants of Aspergillus awamori glucoamylase by
random mutagenesis:
inactivation kinetics and structural interpretation.
Protein Eng.
7, 1005-1012.
Libby, C. B., C. A. G. Cornett, P. J. Reilly,
and C. Ford. (1994) Effect of
amino acid
deletions in the O-glycosylated region of Aspergillus
awamori
glucoamylase. Protein Eng. 7, 1109-1114.
Chen, H.-M., C. Ford, and P. J. Reilly. (1995)
Identification and
elimination
by site- directed mutagenesis of thermolabile aspartyl bonds
in Aspergillus
awamori glucoamylase. Protein Eng. 8, 575-582.
Chen, L., P. Coutinho, Z. Nikolov, and C.
Ford. (1995) Deletion analysis
of the
starch binding domain of Aspergillus glucoamylase.
Protein Eng.
8, 1049-1055.
Chen, H.-M., T. Panda, Y. Li, F. Bühler,
C. Ford, and P. J. Reilly. (1996)
Effect of
replacing helical glycine residues with alanines on the
thermostability
and production of Aspergillus awamori glucoamylase.
Protein Eng.
9, 499-505.
Li, Y., P. J. Reilly, and C. Ford.
(1997) Effect of introducing proline residues
on the stability
of Aspergillus awamori glucoamylase. Protein Eng. 10, 1199-1204.
Fang, T.-Y., P. M. Coutinho,
P. J. Reilly, and C. Ford. (1998) Mutations to
alter Aspergillus
awamori selectivity. I. Y48F, Y116W, Y175F, R241K, S411A
and S411G.
Protein Eng 11, 119-126.
Fang, T.-Y., P. M. Coutinho, P. J.
Reilly, and C. Ford. (1998) Mutations to
alter Aspergillus
awamori glucoamylase selectivity II. Mutation of residues 119
and 121.
Protein Eng 11, 127-133.
Fang, Tsuei-Yun and Clark Ford.
(1998) Protein engineering of Aspergillus awamori
glucoamylase
to increase its pH optimum. Protein Eng. 11, 383-388.
Liu, Hsuan-Liang, Pedro M. Coutinho,
Clark Ford, and Peter J. Reilly. (1998)
Mutations
to alter Aspergillus awamori glucoamylase selectivity III. N20C/A27C,
A27P, S30P,
K108R, K108M, G137A, 311-314 loop, Y312W and S436P.
Protein Eng.
11, 383-398.
Li, Yuxing, Pedro M. Coutinho, and
Clark Ford. (1998) Effect on thermostability and
catalytic
activity of introducing disulfide bonds into Aspergillus awamori
glucoamylase.
Protein Eng.
11, 661-667.
Allen, Martin J., Pedro M. Coutinho,
and Clark F. Ford. (1998) Stabilization of Aspergillus
awamori
glucoamylase
by proline substitution and combining stabilizaing mutations.
Protein Eng.
11, 783-788.
Liu, Hsuan-Liang, Yann Doleyres, Pedro
M. Coutinho, Clark Ford, and Peter J. Reilly (2000)
Replacement
and deletion mutations in the catalytic domain and belt region of Aspergillus
awamori glucoamylase
to enhance thermostability. Protein Eng. 13, 655-659.
Khan, Saber M., Peter J. Reilly, and
Clark Ford (2000) Thermal and Molecular Characterization
of Aspergillus
awamori Glucoamylase Catalytic and Starch?Binding Domains.
Starch/Stärke
52, 385-397
Allen, Martin J., Erica B. Fuchs, and
Clark F. Ford (2002). Asp238®Asn creates a novel
consensus
N-glycosylation site in Aspergillus awamori glucoamylase.
Starch/Stärke
54, 385-392.
Review Articles:
Ford, C. F., I. Suominen, and C. E. Glatz.
(1991) Fusion tails for the
recovery and
purification of recombinant proteins.
Protein Expres.
Purif. 2, 95-107.
Glatz, C. E., and C. F. Ford. (1995)
Genetic Engineering to Enhance the
Selectivity
of Protein Separations.
Appl. Biochem.
Biotechnol. 54, 173-191.
Ford, C.F. (1999) Improving operating
performance of glucoamylase by mutagenesis.
Current Opinion
in Biotechnology 10, 353-357.
Bioethics:
Ford, C. (1996) The Ethics of
Research. Ethos (ISU) v. 42, p. 30.
Reprinted
in: Research and Graduate Education (ISU) v. 8, No. 6, p. 1.
Ford, C. (2000) Genetically Modified Foods: Curse or Blessing? Sowel Review 13, 17-20.