Tools and Resources

Please contact Adam Bogdanove for more information and to obtain materials

Plasmid vectors:

vectors:

Gateway® compatible LexA yeast two hybrid vectors
> pEG202GW
> pJG4-5GW

Gateway® entry vector for blunt end cloning
> pSLR5

Gateway® compatible, broad host range vectors for expression, promoter trapping, and type III effector mining in plant pathogenic bacteria
> pKEB31 (pDD62::GWRfB Km^s Tc^r) for expression
> pKEB32 (pDD62::GWRfB::'avrBs2 Km^s Tc^r) for translocation reporter based effector mining

Gateway® Rice cDNA libraries:

Two treatments: Xoc inoculation, Xoo inoculation
Five timepoints: 2, 4, 8, 24, and 96 hrs. post inoculation
Ten libraries
Average size (number of clones): 5.4 x 10^6
Average Insert Size: 1.3 kb
Insert size range: 0.2 – 3.6 kb

updated July 29, 2005