Physiology -- Animal Biochemistry


QP 601 Enzymes. General Works, Treatises, and Textbooks

EC Enzyme Database

The EC Enzyme Database is a part of the Johns Hopkins University BioInformatics Web Server and is an enzyme classification database.
Within the site's main page, the user will find a link to search the database, a keyword search field, as well as general site information and searching instructions. Likewise, a link is provided to a user's manual for the site; here, users will find information discussing the general scope of the database, contact information for database entries, as well as specifications for the structure, scope, and form of database entries.
To search the database, a user should submit data to the keyword field or select the "search" link. Each entry within the database contains information concerning EC characterized enzymes, with information addressing the EC number, recommended enzyme name, any alternative names, catalytic activity, cofactors, pointers to SWISS-PROT entries that correspond to the enzyme, and pointers which note disease(s) associated with a deficiency of the enzyme. The actual entry for an enzyme will be in a list form, each line assigned an abbreviation, noted in parentheses within the following list. Data presented in the final form consists of: (1) identification (ID); (2) description [official name] (DE); (3) alternate name(s) (AN); (4) catalytic activity (CA); (5) cofactors (CF); (6) comments (CC); (7) associated diseases (DI); and (8) cross references to SWISS-PROT (DR). The symbol "//" denotes the termination line of an entry.
The site is sponsored by the Johns Hopkins University BioInformatics Web Server, a division of the Genome Database; Johns Hopkins University School of Medicine; Baltimore, Maryland.
To Search:
Select "search" link and fill in keyword field. Scroll to view matching entries. Consult user's manual for description of entry contents.

Enzyme Nomenclature Database

The Enzyme Nomenclature Database (ENZYME) is a "...repository of information relative to the nomenclature of enzymes. It is primarily based on the recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUBMB) and it describes each type of characterized enzyme for which an EC (Enzyme Commission) number has been provided."
Upon accessing the site, the user will find a site disclaimer and a link to new additions within the ExPASy server. Also within the main page is the database search page, offering the following fields: (1) enzyme number; (2) enzyme class; (3) description (official name) or alternative name(s); (4) chemical compound; (5) cofactor; and (6) searching within the comments line. Access is also given to database documents: (1) ENZYME user's manual; (2) how to obtain a copy of the database; and (3) report forms for new database entries or for errors/updates within entries. Similarly, a link is provided to the Biochemical Pathways WWW site, a database tool.
Users should select a search method from the main page's options; a query field is then produced, into which data is submitted. Users should then scroll to view the results page, which offers: (1) submitted data; (2) official enzyme name; (3) catalyzed reaction information (with links to further information); and (4) cross references links.
At the bottom of the site's main page are links to other enzyme databases: (1) SWISS-PROT; (2) PROSITE; (3) SWISS-2DPAGE; (4) SWISS-3DIMAGE; and (5) SeqAnalRef. Access is also given to sequence analysis tools, to e-mail the site creator, and to the ExPASy Molecular Biology homepage. The database and its site are maintained by the ExPASy Molecular Biology Server; Geneva University Hospital; the University of Geneva; Switzerland.
To Search:
Fill in search fields within main page; fill in fields within specialized query page. Scroll to view matching results.