BARLEX: Exploring the Barley Genome: anchoring the barley physical map by explorative genomic sequencing consortium by 4 partners: IPK (Nils Stein, PI of the consortium; Uwe Scholz, co-PI), FLI Jena (Mathias Platzer, co-PI), MIPS GSF Munich (Klaus Mayer, co-PI), BAZ Quedlinburg (Frank Ordon, co-PI).

Funding: 6.2 million EURO for 3.5 years, 01.07.07 - 31.12.10, provided by German Ministry of Education and Research (BMBF).

Goals:

• Add 200,000 clones to the HICF project to achieve 12-15x genome coverage, including construction of a third (MboI or PstI) Morex BAC library to complement the current HindIII and EcoRI libraries.
• Sample sequencing of 3000 BACs (BAC pools using barcoded adaptors and 454 technology) to harness gene content information for surveying the barley genome and anchoring of the physical to the genetic map.
• Anchoring 1000 gene or BACend derived markers to the physical map.
• Anchoring DArT markers to the physical map.
• Map based cloning of two virus resistance genes by taking advantage of the barley physical map resources.
• Build bioinformatics environment for integrating barley genomics.