; tocsyt.bf, by B. Fulton, March, 1998. ; TOCSY with water flip-back and WATERGATE ; D. B. Fulton et al, J. Biomol. NMR, _8_ 213-218 (1996). ; Phase-sensitive in F1 via States-TPPI. ; Mixing sequence: TOWNY-16 #include <Avance.incl> #include <Grad.incl> "d11=30m" "d12=20u" "d13=4u" "p2=(p1*2)" "p9=(p1*3.98)" ; p90 for pl10=12 "p10=(p9*.1666)" "p11=(p9*.5)" "p12=(p9*.8333)" "p13=(p9*3.0)" "d0=(in0/2-p1*2/3.14159-d13*2)" "l10=d8/(p9*72)" "l3=td1/2" 1 ze 2 d11*2 3 d11 4 d12 d1 pl1:f1 50u UNBLKGRAD p1 ph1 d13 gron1 d0 d13 groff d13 gron2 d0 d13 groff p1 ph2 d17 gron3 d16 groff d12 pl10:f1 5 (p10 ph22 p12 ph24 p13 ph22 p11 ph24) (p10 ph24 p12 ph22 p13 ph24 p11 ph22) (p10 ph24 p12 ph22 p13 ph24 p11 ph22) (p10 ph22 p12 ph24 p13 ph22 p11 ph24) (p10 ph22 p12 ph24 p13 ph22 p11 ph24) (p10 ph22 p12 ph24 p13 ph22 p11 ph24) (p10 ph24 p12 ph22 p13 ph24 p11 ph22) (p10 ph24 p12 ph22 p13 ph24 p11 ph22) (p10 ph24 p12 ph22 p13 ph24 p11 ph22) (p10 ph22 p12 ph24 p13 ph22 p11 ph24) (p10 ph22 p12 ph24 p13 ph22 p11 ph24) (p10 ph24 p12 ph22 p13 ph24 p11 ph22) (p10 ph24 p12 ph22 p13 ph24 p11 ph22) (p10 ph24 p12 ph22 p13 ph24 p11 ph22) (p10 ph22 p12 ph24 p13 ph22 p11 ph24) (p10 ph22 p12 ph24 p13 ph22 p11 ph24) lo to 5 times l10 d13 d17 gron4 d16 groff p20:sp1 ph12 d13 p20:sp1 ph11 d13 p20:sp1 ph13:r d12 pl1:f1 p1 ph3 d17 gron5 d16 groff d12 p21:sp2 ph14:r d12 p2 ph4 d12 p21:sp2 ph14:r d17 gron5 d16 groff d12 BLKGRAD go=2 ph31 d11 wr #0 if #0 ip1 zd d11 ip11 lo to 3 times 2 d11 id0 lo to 4 times l3 5s exit ph1=0 2 ph2=0 0 0 0 0 0 0 0 2 2 2 2 2 2 2 2 ph3=0 0 2 2 1 1 3 3 ph4=0 0 0 0 1 1 1 1 ph11=2 0 ph12=2 2 2 2 2 2 2 2 0 0 0 0 0 0 0 0 ph13=2 2 0 0 3 3 1 1 ph14=2 2 2 2 3 3 3 3 ph22=0 ph24=2 ph31=0 2 2 0 1 3 3 1 2 0 0 2 3 1 1 3 ;pl1: high power level [0 dB] ;pl10: spin-lock power level [12-15 dB] ;sp1: low power level for selective pulses on water ;sp2: low power level for selective pulse in WATERGATE ;p1 : 90 degree transmitter high power pulse ;p9 : 90 degree spin-lock pulse for mixing ;p20: selective 90 degree flip-back pulse [sp1] ;p21: selective 90 degree pulse on water [sp2] ;d0 : incremented delay ;d1 : relaxation delay; 1-5 * T1 ;d11: delay for disk I/O [30 msec] ;d12: delay for power switching [20 usec] ;d13: short delay (e.g. to compensate delay line) [4 usec] ;d16: settling time after gradient pulse [100 usec] ;d17: gradient pulse duration [500 usec] ;l10: loop for TOWNY-16 cycle: ((p9*72) * l10) = mixing time ;in0: 1/(2 * SW) = DW ;nd0: 2 ;NS: 16 * n ;DS: 16 ;td1: number of experiments ;MC2: States-TPPI