Run a ¹H 1D spectrum with WATERGATE

1. Follow the instructions in Run a 1D spectrum and calibrate 90° pulse width

Note: for this experiment it is important to accurately measure the 90° pulse width.

2. Create a new experiment

Type edc to create a new experiment. Enter desired values for NAME and EXPNO. Set USER to your user name and DU=u, PROCNO=1, TYPE=nmr.

Type rpar Stan1Dwg to load a standard parameter set. Press COPY ALL. Type ii to initialize the instrument.

3. Acquire and process a spectrum

Type zg to acquire an FID.

Note: the lock signal will bounce up and down as the field gradients are pulsed. This is normal.

Wait for the checklockshift:finished message.

Type ef to perform exponential multiplication and Fourier transformation.

Type apk to automatically phase the spectrum.

Note: more detailed instructions on processing are available in Process a 1D spectrum.

4. Optimize parameters

Adjust the sweep width to accomodate the chemical shift range of your sample by typing sw and entering a new value in ppm.

If the signal-to-noise ratio is too low increase the number of scans by typing ns and enter a new value, which should be a multiple of 8.

If the spectrum contains spikes or the baseline is wavey the receiver gain may be too high. Type rg and enter a smaller value.

Optional: you can automatically optimize the receiver gain by typing rga.

Note: if you change rg you will have to rephase the spectrum.

Reacquire and process a new spectrum by typing zg, then efp.