ISU B/B NMR Facility: NOESY with WATERGATE

NOESY with water flip-back and WATERGATE

Description 2D NOESY with bipolar gradients during evolution, water flip-back prior to read-out pulse, and WATERGATE solvent suppression. Employs magic angle field gradients.

References G. Lippens et al, J. Biomol. NMR, 5, 327-331 (1995).
D. B. Fulton and F. Ni, J. Magn. Reson., 129, 93-97 (1997).

Pulse Sequence noesyfl.bf

Parameter Set noesyfl.bf

Parameters to be Adjusted p1, ns, d1, d8, sp1, phcor13, sp2, phcor14

Description	2D NOESY with bipolar gradients during evolution, water flip-back prior to read-out pulse, and WATERGATE solvent suppression. Employs magic angle field gradients.
References	G. Lippens et al, J. Biomol. NMR, 5, 327-331 (1995). D. B. Fulton and F. Ni, J. Magn. Reson., 129, 93-97 (1997).
Pulse Sequence	noesyfl.bf
Parameter Set	noesyfl.bf
Parameters to be Adjusted	p1, ns, d1, d8, sp1, phcor13, sp2, phcor14

Instructions

Acquire a 1D data set to determine SW. Calibrate p1 (¹H 90° pulse at pl1=0, 9 to 13 µs).
Create a 2D experiment and read in parameter set.
Set p1. Adjust d1, d8 (mixing time, 50 to 500 ms), 1 sw = 2 sw and ns as required for your sample.
Set pulprog = noe_setup.bf. Set rg = 128. In gs mode adjust sp1 and phcor13 iteratively to optimize the water flip-back pulse, i.e, minimize the FID.
Reset pulprog = noesyfl.bf. Set rg = 1024. In gs mode adjust sp2 and phcor14 iteratively until WATERGATE is optimized.