ISU B/B NMR Facility: DQF-COSY with WATERGATE

DQF-COSY with WATERGATE

Description Gradient-enhanced phase-sensitive DQF COSY with WATERGATE solvent suppression incorporated into a refocusing delay. Employs magic angle field gradients.

References L. A. Trimble and M. A. Bernstein, J. Magn. Reson. B, 105, 67-72 (1994).
P. C. M. van Zijl et al, J. Magn. Reson. A, 113, 265-270 (1995).

Pulse Sequence cosygpw.bf

Parameter Set cosygpw.bf

Parameters to be Adjusted p1, d1, ns, sp1, phcor11

Description	Gradient-enhanced phase-sensitive DQF COSY with WATERGATE solvent suppression incorporated into a refocusing delay. Employs magic angle field gradients.
References	L. A. Trimble and M. A. Bernstein, J. Magn. Reson. B, 105, 67-72 (1994). P. C. M. van Zijl et al, J. Magn. Reson. A, 113, 265-270 (1995).
Pulse Sequence	cosygpw.bf
Parameter Set	cosygpw.bf
Parameters to be Adjusted	p1, d1, ns, sp1, phcor11

Instructions

Acquire a 1D data set to determine SW. Calibrate p1 (¹H 90° pulse at pl1=0, 9 to 13 µs).
Create a 2D experiment and read in parameter set.
Set p1. Adjust d1, 1 sw = 2 sw and ns as required for your sample.
In gs mode adjust sp1 and phcor11 iteratively until optimal solvent suppression is attained.