Resources for Users

Disclaimer. These documents are intended for local users of the ISU Biomolecular NMR facility. Outsiders are welcome to examine or copy these documents. However, they are presented "as is". There is no warranty as to their functionality or safe operation. Iowa State University is not accountable or liable for any physical or other damage incurred during the use of a pulse program or other information obtained from these documents.

Safety

Procedures for handling data

• Transferring data to another computer

• Backing up data on tape

Reading List

Sample preparation

Introductory NMR experiments

• Setting up

• Gradient auto shimming

• Run a ¹H 1D spectrum

• Run a ¹H 1D spectrum with presaturation

• Run a ¹H 1D spectrum with WATERGATE

• Process a ¹H 1D spectrum

• Chemical Shift Referencing

Advanced NMR experiments

• 2D DQF COSY with WATERGATE

• 2D ¹H-¹H DQ spectroscopy

• 2D NOESY with water flip-back and WATERGATE

• 2D TOCSY with water flip-back and WATERGATE

• ¹H-¹⁵N HSQC optimized for proteins

• ¹H-¹⁵N HSQC for proteins, 3-9-19 version

• 3D ¹H-¹⁵N HSQC-NOESY

• 3D ¹H-¹⁵N HSQC-TOCSY

• 3D ¹H-¹⁵N HMQC-NOESY-HMQC